PROTEINS ASSOCIATED W/ CAVEOLAE FROM ADIPOCYTES

与脂肪细胞小窝相关的蛋白质

• 批准号: 7722974
• 负责人: PAUL F PILCH
• 金额: $ 0.07万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722974
• 关键词: Adipocytes; CD36 gene; Caveolae; Caveolins; Cell membrane; Complement; Computer Retrieval of Information on Scientific Projects Database; Electron Microscopy; Funding; Grant; Institution; Insulin; Insulin Receptor; Label; Link; Lipids; Lipoprotein Receptor; Metabolism; Monoclonal Antibodies; Play; Procedures; Proteins; Regulation; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; caveolin 1; cell type; diamino oxhydrase; flotillin; insulin signaling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adipocytes play an important role in the insulin-dependent regulation of organismal fuel metabolism and express caveolae at levels as high or higher than any other cell type. Recently, a link between insulin signaling and caveolae has been suggested. Nevertheless, adipocyte caveolae have been the subject of relatively few studies, and their contents have been minimally characterized. With the aid of a new monoclonal antibody, we developed a rapid procedure for the immunoisolation of caveolae derived from the plasma membrane of adipocytes, and we characterized their protein content. We find that immunopurified adipocyte caveolae have a relatively limited protein composition, and they lack the raft protein, flotillin, and insulin receptors. Immunogold labeling and electron microscopy of the adipocyte plasma membrane confirmed the lack of insulin receptors in caveolae. In addition to caveolins, the structural components of caveolae, their major protein constituents, are the semicarbazide-sensitive amine oxidase and the scavenger lipoprotein receptor CD36. Ongoing studies that employ mass spectral analysis focus on identifying additional protein components and the lipid complement associated with caveolae.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 脂肪细胞在胰岛素依赖性的有机燃料代谢的调节中起着重要作用，并在比任何其他细胞类型的水平上表现出高度或更高的水平。最近，提出了胰岛素信号传导与小窝之间的联系。然而，脂肪细胞小窝是相对较少的研究的主题，它们的内容的特征很少。借助新的单克隆抗体，我们开发了一种快速的程序，用于从脂肪细胞的质膜中衍生出的口腔免疫化，并表征了它们的蛋白质含量。我们发现免疫促性脂肪细胞小窝的蛋白质组成相对有限，并且缺乏筏蛋白，鞭lotillin和胰岛素受体。脂肪细胞质膜的免疫元标记和电子显微镜证实了小窝中缺乏胰岛素受体。除小窝蛋白外，它们的主要蛋白质成分的小窝结构成分是半北极氮敏感的胺氧化酶和清除剂脂蛋白受体CD36。正在进行的采用质谱分析的研究重点是鉴定其他蛋白质成分和与小窝相关的脂质补充。