MSA1 IS REQUIRED FOR PROPER TIMING OF G1-SPECIFIC TRANSCRIPTION

G1 特异性转录的正确计时需要 MSA1

• 批准号: 7723644
• 负责人: CURT WITTENBERG
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723644
• 关键词: Affect; Binding; Cell Cycle; Computer Retrieval of Information on Scientific Projects Database; Cyclin-Dependent Kinases; Funding; Genes; Genetic Transcription; Grant; Homologous Protein; Institution; Mediating; Merozoite Surface Protein 1; Messenger RNA; Phase; Protein Overexpression; Proteins; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Saccharomycetales; Screening procedure; Source; Time; Transcriptional Activation; Transcriptional Regulation; United States National Institutes of Health; base; novel; promoter; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the budding yeast Saccharomyces cerevisiae, cell cycle initiation is prompted during G(1) phase by Cln3/cyclin-dependent protein kinase-mediated transcriptional activation of G(1)-specific genes. A recent screening performed to reveal novel interactors of SCB-binding factor (SBF) and MCB-binding factor (MBF) identified, in addition to the SBF-specific repressor Whi5 and the MBF-specific corepressor Nrm1, a pair of homologous proteins, Msa1 and Msa2 (encoded by YOR066w and YKR077w), as interactors of SBF and MBF, respectively. MSA1 is expressed periodically during the cell cycle with peak mRNA levels occurring at the late M/early G(1) phase and peak protein levels occurring in early G(1). Msa1 associates with SBF- and MBF-regulated target promoters consistent with a role in G(1)-specific transcriptional regulation. Msa1 affects cell cycle initiation by advancing the timing of transcription of G(1)-specific genes. Msa1 binds to SBF- and MBF-regulated promoters and binding is maximal during the G(1) phase. Binding depends upon the cognate transcription factor. Msa1 overexpression advances the timing of SBF-dependent transcription and budding, whereas depletion delays both indicators of cell cycle initiation. Similar effects on MBF-regulated transcription are observed. Based upon these results, we conclude that Msa1 acts to advance the timing of G(1)-specific transcription and cell cycle initiation.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在酿酒酵母的发芽酵母菌中，CLN3/Cyclin依赖性蛋白激酶介导的G（1）特异性基因的转录激活在G（1）期间引起细胞周期起始。最近进行的筛查揭示了SCB结合因子（SBF）和MCB结合因子（MBF）的新型相互作用，此外还确定了SBF特异性的阻遏物WHI5和MBF特异性核心Pressor NRM1（由MSA1和MSA2相互作用）（由Yor077777777777777777）（YOR077），Yor066W和YOR06W和YOR06W和YOR06W和YOR06W和YOR06W和YOR06W和YOR06W和YOR06W，以及MBF分别。 MSA1在细胞周期期间定期表达，峰值mRNA水平发生在M/早期G（1）早期（1）和峰蛋白水平发生在早期G（1）中。 MSA1与SBF和MBF调节的靶启动子相关联，与G（1）特定的转录调控中的作用一致。 MSA1通过推进G（1）特异性基因转录的时间来影响细胞周期的起始。 MSA1与SBF和MBF调节的启动子结合，在G（1）期间结合最大。结合取决于同源转录因子。 MSA1的过表达促进了依赖SBF的转录和发芽的时间，而耗尽延迟了细胞周期起始的两个指标。观察到对MBF调节的转录的类似作用。基于这些结果，我们得出的结论是，MSA1的作用是为了推动G（1）特异性转录和细胞周期启动的时间。