THE IDENTIFICATION OF SMC-5 ASSOCIATED PROTEINS IN C ELEGANS BY CO-IMMUNOPRECIP

联合免疫法鉴定线虫中 SMC-5 相关蛋白

• 批准号: 7723668
• 负责人: Raymond C Chan
• 金额: $ 0.81万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723668
• 关键词: Adult; Affect; Biological Process; Caenorhabditis elegans; Cells; Chromosomes; Computer Retrieval of Information on Scientific Projects Database; Congenital Abnormality; DNA; Defect; Development; Developmental Process; Ensure; Fertility; Funding; Genome Stability; Genomics; Grant; Human; Human body; Infertility; Institution; Life; Maintenance; Nematoda; Oocytes; Organism; Proteins; Research; Research Personnel; Resources; Soil; Source; United States National Institutes of Health; improved; mouse Smc1l1 protein; mouse Smc1l2 protein; repaired; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The information directing the function of each cell within the human body is encoded in genomic DNA. The proper organization of genomic DNA facilitates the repair of damage to the DNA sustained from environmental insults and to ensure its accurate distribution to every cell within the body. The essential need to organize genomic DNA is shared in many simpler living creatures or organisms. Many of the cellular factors needed to preserve human genomic integrity are also found in these simpler organisms, including a set of factors termed the Structural Maintenance of Chromosomes (or SMC) proteins. The proposed research examines the function for the SMC-5 and SMC-6 proteins in genomic stability. We are examining this vital biological process in the soil-dwelling roundworm, Caenorhabditis elegans, which is ideally suited for studies of cellular and developmental processes. Our preliminary studies in C. elegans have shown the importance for these SMC proteins in promoting genomic stability. Most interestingly, we found evidence for an important requirement for these SMC proteins in the cells that give rise to oocytes and sperms. Defects in SMC-5 and SMC-6 caused infertility in adult worms and increased the likelihood for developmental defects in the offspring from the affected adult worms. Given the conservation of SMC-5 and SMC-6 proteins in human, the information gained from these studies will improve our understanding of how the analogous human proteins may impact human fertility and birth defects.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 指导每个细胞在人体内部的功能的信息是在基因组DNA中编码的。 基因组DNA的适当组织有助于修复环境损害受到的DNA的损害，并确保其准确的分布到体内每个细胞。 组织基因组DNA的基本需求在许多简单的生物或生物中共有。 在这些简单的生物中也发现了维持人基因组完整性所需的许多细胞因素，包括一组称为染色体（或SMC）蛋白结构维持的因素。 拟议的研究研究了基因组稳定性中SMC-5和SMC-6蛋白的功能。 我们正在研究秀丽隐杆线虫的土壤round虫中的这一至关重要的生物学过程，该过程非常适合研究细胞和发育过程。 我们在秀丽隐杆线虫的初步研究表明，这些SMC蛋白在促进基因组稳定性方面的重要性。 最有趣的是，我们发现了对细胞中这些SMC蛋白的重要要求的证据，这些SMC蛋白会引起卵母细胞和精子。 SMC-5和SMC-6的缺陷导致成人蠕虫不孕，并增加了受影响的成年蠕虫后代发育缺陷的可能性。 鉴于人类中SMC-5和SMC-6蛋白的保存，从这些研究中获得的信息将提高我们对类似的人蛋白如何影响人类生育能力和先天缺陷的理解。