YEAST RDNA PROJECT

酵母RDNA项目

• 批准号: 7722239
• 负责人: ERICA Y JACOBS
• 金额: $ 0.55万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722239
• 关键词: Behavior; Binding Proteins; Cells; Chromatin; Computer Retrieval of Information on Scientific Projects Database; Funding; Gene Expression; Genes; Goals; Grant; Growth; Histones; Institution; Location; Maps; Mass Spectrum Analysis; Modeling; Modification; Process; Protein Biosynthesis; Proteins; Research; Research Personnel; Resources; Ribosomal DNA; Ribosomes; Role; Saccharomycetales; Source; Time; United States National Institutes of Health; Yeasts; chromatin protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to study the role of chromatin proteins in gene expression and silencing, we are analyzing chromatin proteins at the ribosomal DNA (rDNA) locus in budding yeast. Ribosomes are required for the fundamental process of protein synthesis, and their expression is regulated according to how much proteins cells need. This in turn depends on how aggressively they are dividing. In wild type yeast growing exponentially, only about half the ~150 tandemly repeated rDNA genes are active at any time, but as growth plateaus all of the repeats become silenced. Since the repeats have the same sequence, differences in behavior between them must be caused by differences in associated factors. This project has three specific aims. First we will purify native rDNA chromatin from yeast cells. Second we will identify the bound proteins, map their locations along the rDNA, and determine how they are modified using mass spectrometry. Third we will determine differences in chromatin composition and modifications between rDNA that is ?on? (transcribed) and rDNA that is off (silent), and describe how their distributions change as the locus is progressively activated or silenced. The ultimate goal is not only a map of active and silenced rDNA chromatin (including histone modifications), but also a dynamic model of rDNA activation and silencing.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 为了研究染色质蛋白在基因表达和沉默中的作用，我们正在分析芽殖酵母中核糖体 DNA (rDNA) 位点的染色质蛋白。核糖体是蛋白质合成的基本过程所必需的，其表达根据细胞需要的蛋白质数量进行调节。这反过来又取决于他们分裂的程度。在呈指数增长的野生型酵母中，大约 150 个串联重复的 rDNA 基因在任何时候都只有大约一半是活跃的，但随着生长平台期，所有重复都会变得沉默。由于重复序列具有相同的序列，因此它们之间的行为差​​异必定是由相关因素的差异引起的。 该项目有三个具体目标。首先，我们将从酵母细胞中纯化天然 rDNA 染色质。其次，我们将识别结合的蛋白质，绘制它们在 rDNA 上的位置，并使用质谱法确定它们是如何被修饰的。第三，我们将确定“on”上的 rDNA 之间染色质组成和修饰的差异。 （转录）和关闭（沉默）的 rDNA，并描述随着基因座逐渐激活或沉默，它们的分布如何变化。最终目标不仅是活性和沉默 rDNA 染色质（包括组蛋白修饰）的图谱，而且是 rDNA 激活和沉默的动态模型。