INTERNATIONAL GENE TRAP CONSORTIUM (IGTC) RESOURCE

国际基因陷阱联盟 (IGTC) 资源

• 批准号: 7723510
• 负责人: THOMAS E FERRIN
• 金额: $ 27.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723510
• 关键词: Alleles; Bioinformatics; Cell Line; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Databases; ES Cell Line; Educational workshop; Funding; Generations; Genes; Genome; Goals; Grant; Human; Institution; International; Laboratories; Libraries; Modification; Mus; Mutagenesis; Mutant Strains Mice; Mutate; Mutation; Organism; Reporter; Reporting; Research; Research Personnel; Resources; Scientist; Site; Source; United States National Institutes of Health; Work; base; embryonic stem cell; genome sequencing; interest; isoglobotriaosylceramide; loss of function; loss of function mutation; mouse genome; tool; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. With the completion of the human and mouse genome sequences, there has been increased interest in developing tools for genome-wide mutagenesis in the mouse, with the goal of analyzing genome function in the context of the intact organism. Gene trapping is a high-throughput approach that can be used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. Gene trap vectors simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA tag for the rapid identification of the disrupted gene. The generation of mutant mice from ES cell lines carrying gene trap insertions could be applied to large-scale functional analysis of mammalian genes. The International Trap Consortium (IGTC) consists of laboratories around the world working together to generate a public library of mutated murine ES cell lines. Such cell lines can be obtained on a non-collaborative basis by scientists interested in generating reporter-tagged, loss-of-function mutations in mice. In addition to loss of function, newer gene trap vectors offer a variety of post-insertional modification strategies to allow for the generation of other experimental alleles. The cooperative goal of the IGTC is to generate an international resource representing all or most genes in the mouse genome, and to provide the bioinformatics and logistical support to make the resource valuable and available to scientists. At the IGTC workshop held at UCSF in April, 2005, the membership of the IGTC agreed to centralize access to all publicly available gene trap lines by developing a user-oriented Website for the IGTC. It was further agreed that there should be a standardized annotation and identification to provide high confidence data for gene trap lines. The IGTC website, database, and annotation and identification pipeline are hosted by the RBVI. Further information on this project is available at http://www.genetrap.org/

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 随着人类和小鼠基因组序列的完成，人们对在小鼠中开发全基因组诱变工具的兴趣增加，目的是在完整生物的背景下分析基因组功能。 基因诱捕是一种高通量方法，可用于在小鼠胚胎（ES）细胞中引入基因组插入插入突变。基因陷阱载体同时突变并报告内源基因在插入部位的表达，并为快速鉴定破坏的基因提供了DNA标签。携带基因陷阱插入的ES细胞系的突变小鼠可以应用于哺乳动物基因的大规模功能分析。 国际陷阱财团（IGTC）由世界各地的实验室组成，共同创建了一个突变的鼠类ES细胞系的公共图书馆。有兴趣在小鼠中产生报道者标记的，功能丧失的突变的科学家可以在非授权的基础上获得此类细胞系。除功能丧失外，较新的基因陷阱向量还提供了各种插入后修改策略，以允许生成其他实验等位基因。 IGTC的合作目标是生成代表鼠标基因组中所有或大多数基因的国际资源，并提供生物信息学和后勤支持，以使资源有价值且可供科学家使用。 在2005年4月在UCSF举行的IGTC研讨会上，IGTC的成员同意通过为IGTC开发以用户为导向的网站来集中对所有公开可用的基因陷阱系列的访问。 进一步同意，应该有标准化的注释和识别，以提供基因陷阱线的高置信度数据。 IGTC网站，数据库以及注释和标识管道由RBVI托管。 有关此项目的更多信息，请访问http://www.genetrap.org/