CRYSTAL STRUCTURES OF BACTERIAL PATHOGEN PROTEINS

细菌病原体蛋白的晶体结构

• 批准号: 7721793
• 负责人: PARTHO GHOSH
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721793
• 关键词: Bacterial Proteins; Borrelia burgdorferi; Cells; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Data; Drug Delivery Systems; Escherichia coli; Funding; Grant; Heparin; Human; Institution; Listeria monocytogenes; Lyme Disease; Membrane Proteins; Microbial Drug Resistance; Multi-Drug Resistance; Oligosaccharides; Order Spirochaetales; Pharmaceutical Preparations; Process; Proteins; Receptor Protein-Tyrosine Kinases; Research; Research Personnel; Resources; Role; Social Welfare; Source; Streptococcus pyogenes; Structure; Tissues; United States National Institutes of Health; Virulence; X ray diffraction analysis; X-Ray Diffraction; base; microbial; novel; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Infectious diseases caused by bacterial pathogens represent a major toll on human welfare. We are determining the structures of microbial pathogen proteins that interact with host cell targets and have a role in virulence. With the emergence of drug-resistant microbial pathogens, the need to identify novel drug targets and novel drugs based on structural information has taken on urgency. We propose to collect SAD X-ray diffraction data on E. coli AcrA, a bacterial protein required for conferring multi-drug resistance; MAD data on Borrelia burgdorferi Bgp, a protein essential to colonization of host tissues by the spirochete that causes Lyme disease; MAD data on Streptococcus pyogenes M1 protein, a surface protein required for virulence of this Gram-positive pathogen; monochromatic data on Listeria monocytogenes InlB soaked with heparin oligosaccharides to determine the mechanism by which this molecule activates the host receptor tyrosine kinase Met during the process of intracellular invasion.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 细菌病原体引起的传染病代表了人类福利的重大损失。 我们正在确定与宿主细胞靶标相互作用并在毒力中起作用的微生物病原体蛋白的结构。 随着耐药性微生物病原体的出现，基于结构信息的新型药物靶标和新型药物的需求已遵循紧迫性。 我们建议在大肠杆菌ACRA上收集SAD X射线衍射数据，这是一种赋予多药耐药性所需的细菌蛋白。关于伯氏伯氏菌BGP的疯狂数据，这是一种导致莱姆病的螺旋体对宿主组织定殖必不可少的蛋白质；关于链球菌为链球菌M1蛋白的疯狂数据，这是该革兰氏阳性病原体毒力所需的表面蛋白；用肝素寡糖浸泡的单核细胞增生李斯特菌的单色数据，以确定该分子在细胞内浸润过程中激活宿主受体酪氨酸激酶遇到的机制。