STUDIES ON ENZYMES INVOLVED IN THE GLOBAL CYCLING OF HYDROGEN, NITROGEN AND CARB

参与氢、氮和碳水化合物全球循环的酶的研究

• 批准号: 7721997
• 负责人: JOHN W PETERS
• 金额: $ 0.65万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721997
• 关键词: Address; Alkenes; Biochemical; Catalysis; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Goals; Grant; Hydrogen; Hydrolysis; Individual; Institution; Ketones; Methods; Nitrogen; Pathway interactions; Production; Reaction; Research; Research Personnel; Resolution; Resources; Source; Structure; United States National Institutes of Health; base; enzyme mechanism; enzyme structure; novel; phosphate ester; programs; research study; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The following program proposal narrative describes individual experiments for several projects that involve the use of synchrotron radiation for structure determination using x-ray diffraction methods to address problems concerning the mechanism of enzyme catalyzed reactions. Toward these ends, we are determining the structure of novel enzymes and utilizing information obtained by previous biochemical and biophysical studies as a basis for capturing and determining the structure of enzymes in defined states that mimic intermediates along the pathway of catalysis. The overall goal of these studies is to utilize the structures of defined states of certain enzymes to contribute to the overall understanding of enzyme mechanism and therefore there is significant value in conducting these studies at high resolution. We are currently pursuing these studies for enzymes involved in hydrogen production, nitrogen reduction, phosphate ester hydrolysis, and bioconversion of alkenes and ketones.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 以下程序提案叙述描述了几个项目的单个实验，这些实验涉及使用X射线衍射方法使用同步加速器辐射来确定结构，以解决有关酶催化反应机制的问题。 在这些目的方面，我们正在确定新型酶的结构，并利用先前的生化和生物物理研究获得的信息，作为在定义的状态下捕获和确定酶沿催化途径的定义状态中的酶结构的基础。 这些研究的总体目标是利用某些酶的定义状态的结构来有助于对酶机制的整体理解，因此在高分辨率下进行这些研究具有重要的价值。 目前，我们正在为参与氢生产，氮还原，磷酸酯水解以及烯烃和酮的生物转化的酶进行这些研究。