CELL SORTER/FLOW CYTOMETRY CORE FACILITY

细胞分选仪/流式细胞术核心设施

• 批准号: 7725250
• 负责人: NICOLA M KOUTTAB
• 金额: $ 26.98万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-05 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725250
• 关键词: 5-(6)-carboxyfluorescein diacetate succinimidyl ester; Cell Separation; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Deposition; Development; Event; Flow Cytometry; Funding; Grant; Green Fluorescent Proteins; Human Resources; Institution; Marrow; PTPRC gene; Population; Research; Research Personnel; Resources; Scanning; Side; Skin; Sorting - Cell Movement; Source; Stem cells; Suspension substance; Suspensions; Time; United States National Institutes of Health; Work; instrument; rho

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell Sorter Core provides expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. John Morgan is the full time stem cell/sorter technician. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. The FACS facility is also equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators work with the core and prepare their cell populations for sorting or analysis. The core personnel routinely separate Lin- Rho(lo)Ho(lo), Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core serves all projects in this COBRE carrying out both separations and analysis work. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 细胞分散器核心提供了骨髓或皮肤干细胞分离的专业知识，并分析了细胞悬浮液中的供体细胞。约翰·摩根（John Morgan）是全日制干细胞/魔力技术员。 该核心具有来自细胞化的Mofio高速细胞分辨率。该分散者能够进行干细胞分离，单细胞沉积和大型事件分析。 FACS设施还配备了FACS扫描和FACS Calibur（BD Instruments）。两台机器都可以充当项目所需的任何分析工作的备份。研究人员与核心合作并准备其细胞群进行分类或分析。核心人员通常将lin-rho（Lo）HO（LO），Lin-Hoechst侧种群和LIN-SCA+ CD45-细胞分析，并分析GFP+或CFDA-SE阳性细胞的种群。该核心为这部毛线的所有项目提供服务，同时进行分离和分析工作。核心还致力于发展新的干细胞分离的发展和增强。