Nanotube and nanogold to gear phi29 DNA packaging motor

纳米管和纳米金齿轮 phi29 DNA 包装电机

• 批准号: 7245847
• 负责人: PEIXUAN GUO
• 金额: $ 25.93万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7245847
• 关键词: Bacteriophages; Binding; Biological; Biology; Biomimetics; Chemicals; Complex; DNA; DNA Packaging; DNA Sequence; Data Storage and Retrieval; Devices; Diagnosis; Disease; Disruption; Drug Delivery Systems; Engineering; Facility Construction Funding Category; In Vitro; International Aspects; Link; Mechanics; Medicine; Membrane; Modification; Motor; Movement; Nanotechnology; Nanotubes; Organism; Pattern; Phase; Process; Protein Array; Pump; RNA; Recombinant Proteins; Recombinants; Route; Signal Transduction; Staging; Structure; System; chemical group; density; design; dimer; in vivo; infancy; molecular sieving; nanoGold; nanodevice; nanomachine; nanomaterials; nanometer; nanoparticle; nanoscale; novel; pathogen

DESCRIPTION (provided by applicant): Nanotechniques involve the creation, characterization, and modification of organized nanomaterials to serve as building blocks for the construction of large-scale devices and systems. Living systems contain a wide variety of nanomachines and highly ordered structures, including motors, arrays, pumps, membrane cores, and valves. The novelty and ingenious design of bacterial virus phi29 DNA packaging motor, the strongest biomotor studied to date, have inspired the synthesis of this motor and its components as biomemitics for nanodevices. This 30 nm nanomotor uses six ATP-binding pRNA molecules to gear the motor. An imitative motor has been successfully constructed using purified recombinant proteins and artificially synthesized RNA. It can be turned on and off at will. The formation of ordered structural arrays of the motor complex and its components, the retention of motor function after 3'-end extension of the pRNA, and the ease with which pRNA dimers and trimers can be manipulated combine to make the RNA-containing motor a viable option as mechanical parts in nanotechnology. The long-term objective is to utilize this synthesized motor, together with arrays of its components, as parts in nanodevices, with several major applications: 1) To use this artificial motor as parts for nanodevices, such as apparatuses for in vivo drug delivery. 2) To use the ordered arrays of motor components as building facades for large-scale supramolecular structures to serve as molecular sieves, chips for the diagnosis of diseases, or as ultrahigh density data storage systems. The presence of six pRNA subunits in the hexameric building blocks will allow for the construction of arrays with multiple functions, for example to allow for the separate identification of multiple pathogens. 3) To develop this nanomotor into a DNA-sequencing apparatus, since the DNA-packaging process involves movement of the DNA through a 3.6-nm pore surrounded by six RNA that can be modified to accept chemical signals. Though all of these long-term applications are becoming more and more realizable, practical nanotechnology is still in its infancy. It would be unrealistic to propose applying the phi29 motor system directly to nanodevices in only three or four years. Thus, the short-term objective of this proposal is to construct pRNA or protein arrays that will serve as templates for the construction of patterned supramolecular structures, and to find the best routes through which to link nanotubes and nanogolds as well as to attach biological moieties and chemical groups to the motor or the constructed arrays, pRNA dimers, trimers and hexamers will be used for array construction. Chief among early concerns will be avoiding disruption of the assembly and functioning of the motor and/or arrays due to the incorporation of foreign components. This phase of the study will pave the way toward direct and practical technological applications of the motor and its arrays in nanodevices.

描述（由申请人提供）：纳米技术涉及有组织的纳米材料的创建、表征和修饰，以作为构建大规模设备和系统的构建块。生命系统包含各种各样的纳米机器和高度有序的结构，包括电机、阵列、泵、膜芯和阀门。细菌病毒phi29 DNA包装马达是迄今为止研究的最强的生物马达，其新颖而巧妙的设计激发了这种马达及其组件作为纳米器件仿生学的合成。这个 30 nm 纳米电机使用六个 ATP 结合 pRNA 分子来驱动电机。使用纯化的重组蛋白和人工合成的RNA成功构建了模仿电机。它可以随意打开和关闭。运动复合体及其组件的有序结构阵列的形成、pRNA 3'端延伸后运动功能的保留以及 pRNA 二聚体和三聚体易于操作的结合使包含 RNA 的运动成为作为纳米技术机械零件的可行选择。 长期目标是利用这种合成电机及其组件阵列作为纳米设备的部件，具有以下几个主要应用：1）使用这种人造电机作为纳米设备的部件，例如用于体内药物输送的装置。 2）使用有序的电机组件阵列作为大规模超分子结构的建筑立面，用作分子筛、疾病诊断芯片或超高密度数据存储系统。六聚体构件中存在六个 pRNA 亚基将允许构建具有多种功能的阵列，例如允许单独识别多种病原体。 3) 将这种纳米电机开发成 DNA 测序装置，因为 DNA 包装过程涉及 DNA 穿过 3.6 nm 孔的运动，该孔被 6 个 RNA 包围，这些 RNA 可以被修改以接受化学信号。 尽管所有这些长期应用都变得越来越可行，但实用的纳米技术仍处于起步阶段。提议在短短三四年内将phi29电机系统直接应用于纳米设备是不现实的。因此，该提案的短期目标是构建 pRNA 或蛋白质阵列，作为构建图案化超分子结构的模板，并找到连接纳米管和纳米金以及附着生物部分的最佳途径和化学基团到马达或构建的阵列、pRNA二聚体、三聚体和六聚体将用于阵列构建。早期关注的主要问题是避免由于引入外国组件而导致电机和/或阵列的组装和功能中断。这一阶段的研究将为电机及其阵列在纳米器件中的直接和实际技术应用铺平道路。