A THERAPEUTIC DNA VACCINE IN NONHUMAN PRIMATE AGAINST CHAGAS DISEASE

针对非人类灵长类动物的恰加斯病治疗性 DNA 疫苗

• 批准号: 7562393
• 负责人: ERIC DUMONTEIL
• 金额: $ 1.4万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562393
• 关键词: Adverse effects; Animals; Blood; Brazil; Cardiac; Cessation of life; Chagas Disease; Chronic Phase of Disease; Colon; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Vaccines; Disease; Ear; Esophageal; Funding; Genus Cola; Grant; Heart failure; Individual; Infection; Institution; Liver; Mexico; Nitrofurans; Parasites; Patients; Pharmaceutical Preparations; Pilot Projects; Polymerase Chain Reaction; Primates; Research; Research Personnel; Resources; Risk; Schedule; Skeletal Muscle; Source; Spleen; Tail; Testing; Therapeutic; Tissues; Trypanosoma; United States National Institutes of Health; Vaccines; Week; Whole Blood; Work; day; nonhuman primate; vaccination schedule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Chagas disease, or American Trypanosomiasis, is caused by the protozoan parasite Trypanosoma cruiz. An estimated 16-18 million people are infected and close to 100 million people are at risk of infection from southern Brazil to Mexico. During the chronic phases of the disease, infected individuals undergo extensive tissue damage, primarily cardiac tissue but also spleen, liver, skeletal muscle, colon and esophageal with this tissue damage progressively leading to cardiac failure and death. Over 20,000 deaths per year are attributed to this disease. Treatment of infected patients relies on nitrofurans but these drugs are of little efficacy and have serious side effects. Nine animals (n=6 on Pilot project and n=3 on Gorgas) were inoculated subcutaneously on 1/17/07 with T.cruzi parasites supplied by Dr. Yabsley at UGA. Ear/tail blood smears have been collected weekly since inoculation. To date, no parasites have been found in these smears. Beginning three weeks ago, whole blood has been sent to Dr. Dorn at Loyola to be tested for parasite DNA. PCR results were not clean but five animals have consistently shown bands positive for T.cruzi DNA. I am working on sending her na¿ve blood to determine if the other bands are primate DNA or some other contamination. Meanwhile, none of the scheduled vaccinations have occurred. A total of three DNA vaccines spaced 30 days apart were scheduled to begin last month.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 Chagas病或美国锥虫病是由原生动物寄生虫锥虫巡游引起的。估计有16-1800万人感染，近1亿人有从巴西南部到墨西哥感染的风险。在疾病的慢性阶段，感染的个体受到广泛的组织损伤，原发性心脏组织，但还会受到囊，肝脏，骨骼肌，结肠和食管造成的，逐渐导致心脏衰竭和死亡。每年超过20,000人死亡归因于这种疾病。感染患者的治疗依赖于硝基呋喃，但这些药物的有效性很小，副作用严重。 在1/17/07年1/17/07下，Yabsley博士在UGA提供的T.Cruzi寄生虫，在1/17/07中皮下接种了9只动物（n = 6个动物，n = 3）。自接种以来，每周收集耳/尾血涂片。迄今为止，在这些涂片中尚未发现寄生虫。从三周前开始，全血被送到洛约拉的多恩博士，以测试寄生虫DNA。 PCR结果不是干净的，但是五只动物始终显示出对T.cruzi DNA呈阳性的带。我正在努力发送她的血液，以确定其他乐队是私人DNA还是其他污染。同时，没有预定的疫苗发生。上个月总共有30天的分隔30天的DNA疫苗。