PROPAGATION OF MONKEY MODELS OF HUMAN DISEASE

人类疾病的猴子模型的传播

• 批准号: 7561880
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 22.59万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561880
• 关键词: Alleles; Animals; Assisted Reproductive Technology; Biomedical Research; Breeding; Communities; Computer Retrieval of Information on Scientific Projects Database; Funding; Genotype; Grant; Histocompatibility Antigens Class I; Institution; Macaca mulatta; Modeling; Monkeys; Oocytes; Population; Research; Research Personnel; Resources; Source; Specific qualifier value; Technology; United States National Institutes of Health; Wild Animals; human disease; male; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is currently a significant need for populations of animals with specified genotypes, which cannot be satisfied by the importation of animals from the wild, or by the identification and propagation of valuable founder animals by selective breeding. Indian-origin, rhesus macaques carrying the major histocompatability complex (MHC) class 1 allele, A*01, are particularly needed for vaccine development research. The objective of this research is to demonstrate that the assisted reproductive technologies (ARTs) can be applied to the rapid, efficient propagation of a valuable founder animal using existing technology, thereby establishing an effective approach to satisfying animal requirements of the biomedical research community. The rationale for focusing on a homozygous, founder male is that all offspring produced by this animal will be Mamu-A*01 positive. Moreover, if the heterozygous carriers of the A*01 allele are used as oocyte donors, 50% of the offspring will be homozygous for the allele, creating additional founder animals.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目前对具有特定基因型的动物种群存在巨大需求，而这种需求无法通过从野生进口动物或通过选择性育种来鉴定和繁殖有价值的始祖动物来满足。 疫苗开发研究特别需要携带主要组织相容性复合物 (MHC) 1 类等位基因 A*01 的印度恒河猴。 本研究的目的是证明辅助生殖技术（ART）可以利用现有技术快速、高效地繁殖有价值的始祖动物，从而建立一种有效的方法来满足生物医学研究界的动物需求。 关注纯合的创始人雄性的理由是，该动物产生的所有后代都将呈 Mamu-A*01 阳性。 此外，如果将 A*01 等位基因的杂合携带者用作卵母细胞供体，则 50% 的后代将是该等位基因纯合的，从而产生额外的创始人动物。