Public KIR Database

公共 KIR 数据库

• 批准号: 7550555
• 负责人: Jeffrey S. Miller
• 金额: $ 9.31万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7550555
• 关键词: Alleles; Allogenic; Biological Assay; Cell Transplantation; Chairperson; Classification; Collaborations; Communities; Complementary DNA; Computer software; DNA; DNA Sequence Databases; Data; Databases; Detection; Donor person; EMBL Nucleotide Sequence Database; Education; Electronics; Evaluation; Exons; Genbank; General Practitioners; Genes; Genetic Polymorphism; Genomics; Genotype; Haplotypes; Hematopoietic; Individual; Information Dissemination; Internet; Introns; Killer Cells; Laboratories; Laboratory Study; Literature; Names; Natural Killer Cells; Nomenclature; Numbers; Outcome; Participant; Polymerase Chain Reaction; Principal Investigator; Publishing; Receptor Gene; Research; Resource Informatics; Resources; Retrieval; Sampling; Secure; Sequence Alignment; Site; Software Tools; System; Testing; Time; Uncertainty; Variant; World Health Organization; Writing; base; data mining; member; novel; programs; receptor; tool; web based interface

This application aims to establish a core informatics resource for the Killer-cell ImmunogTobulin-like Receptors (KIR) research community and create an essential tool for the collaborations associatedwith the integrated program on "NK cells their receptors and unrelated donor transplant". The applicant will generate and develop a publicly accessible database of KIR cDNA and genomic sequences; (of which tnere are currently in excess of 100 known) derived from the literature, generalist DNA sequence databases and from direct submission to the KIR sequence database. Software tools will be developed for the direct submission, analysis, manipulation and retrieval of sequences; laboratory based assays for KIR genotyping will be standardized; naplotype and genotype data will be generated and integrated into the database Laboratory studies will also be carried out to better characterize and extend existing KIR allele sequences, and to validate published or submitted sequences. The applicant is Rapporteur and Chairman of IUIS Subcommittee on KIR nomenclature under the auspices of the WHO Nomenclature Committee for factors of the HL.A system. The database will be developed on a Unix platform based around software written by Oracle so as to maintain compatibility with other public databases. Tools for sequence handling will be developed using PERL and will be based on similar tools established previously for handling HLA sequences. The database will be made freely available to users via the world wide web Data-mining of sequence repositorieswill require systematic data-cleaning and confirmation of sequence identity before alleles can be formally named. In addition to standardized KIR sequence alignments, KIR sequence files will also be madeavailable in a number of different formats including FASTA, PIR and MSF for remote download ng by ftp. In time this resource will help in determining the significance of KIR gene variation to the outcomes of allogeneic hematopoietic cell transplantation.

该应用程序旨在建立杀伤细胞免疫球蛋白样的核心信息学资源 受体（KIR）研究社区，并为与受体（KIR）相关的合作创建了一个重要工具 关于“NK细胞及其受体和无关供体移植”的综合计划。申请人将生成 开发一个可公开访问的 KIR cDNA 和基因组序列数据库； （其中有 目前已知超过 100 个）源自文献、通用 DNA 序列数据库和 直接提交至 KIR 序列数据库。将开发用于直接提交的软件工具， 序列的分析、操作和检索；基于实验室的 KIR 基因分型测定将 标准化；将生成基因型和基因型数据并将其集成到数据库实验室中 还将进行研究以更好地表征和扩展现有的 KIR 等位基因序列，并 验证已发布或提交的序列。申请人为IUIS小组委员会报告员兼主席 在世界卫生组织 HL.A 因子命名委员会的支持下，关于 KIR 命名的研究 系统。 该数据库将基于Oracle编写的软件在Unix平台上开发，以便 保持与其他公共数据库的兼容性。序列处理工具将使用以下方式开发 PERL 将基于之前为处理 HLA 序列而建立的类似工具。数据库 将通过万维网免费向用户提供序列存储库的数据挖掘将 在正式确定等位基因之前，需要系统的数据清理和序列同一性确认 命名。除了标准化的 KIR 序列比对之外，还将提供 KIR 序列文件 有多种不同的格式，包括 FASTA、PIR 和 MSF，用于通过 ftp 进行远程下载。及时这个 资源将有助于确定 KIR 基因变异对同种异体结果的重要性 造血细胞移植。