Core-Animal Core

核心-动物核心

• 批准号: 7219434
• 负责人: Laureano D Asico
• 金额: $ 10.48万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219434
• 关键词: angiotensin receptor; animal breeding; animal colony; biomedical facility; blood pressure; clinical research; dopamine receptor; familial hypertension; genetically modified animals; laboratory mouse; telemetry

The mice (D1 and D3 receptor mutants and GRK4 wild type and A142V transgenes) required for project by Jose in this PPG will be bred and maintained by the Animal Core. Colonies to support adult mice of 8-14 weeks will be developed from initial breeders. Heterozygous (+/-) mice will be bred to provide homozygous (+/+) wild type, heterozygous (+/-) and homozygous (-/-) deleted mice in the same litter. The Animal Core has in place functioning breeding colonies and has a detailed plan that specifies the number of mice necessary to support the proposed research. The Core has the infrastructure in place to maintain breeding information in a database that catalogues ear tag identification and breeding history and numerous other parameters. The Animal Core will collect blood and/or tail tissue from weanling mice for genotyping in the Analytical Core. The Animal Core will also measure the conscious mean arterial blood pressure directly by radio-telemetry in a subset of each single-gene deleted or transgenic strain. The Animal Core will provide all technical support for all protocols involving animals and assure that the PPG is in compliance with all institutional regulations regarding animal breeding and experimental use.

Jose 在此 PPG 项目中所需的小鼠（D1 和 D3 受体突变体以及 GRK4 野生型和 A142V 转基因）将由 Animal Core 饲养和饲养。将从最初的饲养者中培育出支持 8-14 周成年小鼠的群体。将培育杂合（+/-）小鼠，以在同一窝中提供纯合（+/+）野生型、杂合（+/-）和纯合（-/-）缺失小鼠。动物核心已经建立了功能齐全的繁殖群，并制定了详细的计划，规定了支持拟议研究所需的小鼠数量。核心拥有适当的基础设施，可以在数据库中维护育种信息，该数据库对耳标识别和育种历史以及许多其他参数进行分类。这 Animal Core 将从断奶小鼠身上收集血液和/或尾部组织，用于在 分析核心。动物核心还将通过无线电遥测技术直接测量每个单基因删除或转基因菌株的子集的意识平均动脉血压。 Animal Core 将为涉及动物的所有协议提供所有技术支持，并确保 PPG 符合有关动物育种和实验使用的所有机构法规。