The Stem Cell Center at The Burnham Institute

伯纳姆研究所干细胞中心

• 批准号: 6964568
• 负责人: EVAN Y SNYDER
• 金额: $ 106.07万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6964568
• 关键词: Stem; Cell; Center; Burnham; Institute

DESCRIPTION (provided by applicant): Human Embryonic Stem Cells (hESC) have remarkable potential for studies of fundamental human developmental biology. The principal goal of this project is to establish the Burnham Stem Cell Center to facilitate collaborative research in the basic biology of hESC and encourage new researchers to enter the field. The Center will enable new and established investigators to develop the utility of hESC as a model system for a diverse range of biological and medical problems. The proposal for the Center has two specific aims. For Aim 1, we will establish a shared core laboratory that provides state-of-art equipment and expert support for area investigators and training in hESC methods. Core programs will be tightly focused on specific goals to provide technical support and expertise, develop new technologies, and train new investigators. The Core Programs are (1) Cell Culture and Maintenance, (2) Cell Characterization, (3) High Throughput Analysis Technology Development, and (4) Data Sharing and Training. These programs will use NIH Registry Cell lines WA01, WA09, and WA14. To encourage new investigators and collaborations, the Center will share responsibility for an annual ten-day NIH-sponsored T15 training course in hESC technology, provide 2-3 day training courses for visiting scientists, sponsor a website for sharing information about hESC, host scientific and ethics symposia twice a year, and support the monthly meetings of the Southern California Stem Cell Consortium. Aim 2 is to sponsor competitive pilot projects that take advantage of the unique potential of hESC. We will support four early-phase, hypothesis-driven pilot projects that address fundamental questions of hESC biology. These pilot projects include investigations of chemical inducers of differentiation, epigenetic controls of differentiation, development of novel high-resolution real time imaging technology to study hESCs in vitro, and molecular and developmental controls of hESC self-renewal.

描述（由申请人提供）：人类胚胎干细胞（HESC）具有显着的人类发育生物学研究的潜力。该项目的主要目标是建立伯纳姆干细胞中心，以促进hESC基本生物学的合作研究，并鼓励新研究人员进入该领域。该中心将使新的和既定的研究人员能够开发hESC作为各种生物学和医学问题的模型系统的实用性。该中心的提议有两个具体的目标。对于AIM 1，我们将建立一个共享的核心实验室，该实验室为区域调查人员提供最先进的设备和专家支持，并在HESC方法中培训。核心计划将紧密关注特定目标，以提供技术支持和专业知识，开发新技术并培训新的研究人员。核心程序是（1）细胞培养和维护，（2）细胞表征，（3）高吞吐量分析技术开发以及（4）数据共享和培训。这些程序将使用NIH注册表WA01，WA09和WA14。为了鼓励新的研究人员和合作，该中心将对hESC技术的每年为期十天的NIH赞助T15 T15培训课程负责，为访问科学家提供2-3天的培训课程，赞助一个网站，以共享有关HESC，主机科学和伦理学聊天室的信息，每年两次，并支持南加州Cellia Cellia Cellia Cellia Cell Cansort的每月会议。 AIM 2是赞助竞争性试点项目，以利用HESC的独特潜力。我们将支持四个早期的假设驱动的试点项目，这些项目解决了HESC生物学的基本问题。这些试点项目包括对分化化学诱导者的研究，分化的表观遗传控制，开发新型的高分辨率实时成像技术，以研究hESC，以及hESC自我恢复的分子和发育控制。