Interjoint coordination and compensation in rat locomotion using x-ray kinematics

利用 X 射线运动学研究大鼠运动的关节间协调和补偿

基本信息

批准号：
7435241
负责人：
Young-Hui Chang
金额：
$ 15.74万
依托单位：
GEORGIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-01 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7435241
关键词：
Accounting Ankle Area Behavior Behavioral Biological Assay Biomechanics Biomedical Research Communities Complex Condition Data Set Development Disease model Exhibits Felis catus Financial compensation Foundations Gait Gait abnormality Genetic Goals Gold Hindlimb Human Image Injury Joints Kinesiology Knock-out Leg Length Lesion Limb structure Literature Locomotion Mammals Maps Measurement Measures Methods Modeling Motor Movement Mus Muscle Muscle denervation procedure Nature Nervous system structure Neuromechanics Optics Pattern Peripheral nerve injury Plantaris muscle Positioning Attribute Range Rat-1 Rattus Recovery Relative (related person)Research Research Personnel Rodent Rodent Diseases Skeleton Skin Soleus Muscle Source Speed Spinal cord injury Standards of Weights and Measures System Technology Testing Time Torque Work base design functional outcomes kinematics limb movement locomotor deficit motor deficit mouse model nerve injury new technology sciatic nerve sensory feedback

项目摘要

DESCRIPTION (provided by applicant): The rat has come to the forefront of many nerve injury models and will likely be associated with the next greatest biomedical breakthroughs in the areas of spinal cord injury and peripheral nerve injury. Groundbreaking methods for studying specific disease models in rodents are increasingly prevalent in the biomedical research community. However, the ability to quantitatively and mechanistically resolve locomotor functional outcomes to test more subtle and sophisticated hypotheses is currently unavailable and must also be further developed. Measuring whole limb movement patterns (kinematics) with skin markers to quantify locomotor function is often the gold standard in other areas of movement science. However, this can present a problem when studying small mammals like rats due to the large errors attributed to movement of the skin relative to the underlying skeleton. The objectives of this project are to develop and test a high-speed x-ray kinematics system for quantifying locomotor deficits in rat hindlimb coordination after specific peripheral nerve injuries. Achieving this objective will provide two immediate, deliverable end-products that will impact areas of biomedical research concerned with quantifying locomotor behavior in rats: (Aim 1) development of an x-ray kinematics locomotor assay that will provide a gold standard for rat locomotor patterns and provide context for the more common skin marker kinematics methods; and, (Aim 2) a theoretical foundation for understanding basic principles of locomotor compensation after specific neuromuscular injuries such as a muscle denervation. The long-term goals of this project are to provide a means to accurately study the different contributions of short-term compensation, long-term compensation and sensory feedback to the control of locomotion after nerve injury. In advancing the study of locomotor function in rats, the results of this project could easily be applied to mouse locomotion and have great implications for the study of locomotion in the hundreds of genetic knockout mouse models. This work will generate technology capable of accurately quantifying motor deficits that map to subtle neuromuscular lesions and form a theoretical basis for studying the mechanisms that drive recovery in more complex lesions such as sciatic nerve injury or spinal cord injury, with eventual applicability to genetically modified rats and mice. Rats and mice are overwhelmingly the research model of choice to study and develop therapies for spinal cord injury and other serious, debilitating insults to the nervous system. Currently the ability to relate specific neuromuscular injuries to specific biomechanical gait deficits in rats does not exist, so the scientific community can only make general conclusions about the efficacy of potential treatments. This project: (1) will generate technology capable of accurately quantifying biomechanical gait deficits that relate to very specific neuromuscular injuries, and (2) generate a theoretical basis for understanding the neuromechanical compensation mechanisms in more complicated injuries such as spinal cord injury and potential for application to genetic causes of gait disorders.

描述（由申请人提供）：大鼠已经走到许多神经损伤模型的最前沿，并且很可能与脊髓损伤和周围神经损伤领域下一个最大的生物医学突破有关。在生物医学研究界，研究啮齿动物特定疾病模型的开创性方法越来越普遍。但是，目前无法使用定量和机械能力解决运动功能结果以测试更微妙和复杂的假设的能力，这也不可以进一步发展。用皮肤标记来测量整个肢体运动模式（运动学）来量化运动功能，通常是运动科学其他领域的黄金标准。但是，由于皮肤相对于基础骨架的运动造成的大误差，因此研究小鼠（例如大鼠）时可能会出现问题。该项目的目标是开发和测试高速X射线运动学系统，以量化特定的外周神经损伤后大鼠后肢协调中的运动缺陷。实现这一目标将提供两种直接可交付的最终产物，这些产品将影响与量化大鼠运动行为的生物医学研究领域：（目标1）开发X射线运动学运动测定法，该测定法将为大鼠运动模式提供金色标准，并为更常见的常见的皮肤标记Kinematics方法提供背景；（目标2）理论基础，用于理解特定神经肌肉损伤（例如肌肉神经神经）之后运动补偿的基本原理。该项目的长期目标是提供一种方法，以准确研究短期补偿，长期补偿和对神经损伤后运动控制的感觉反馈的不同贡献。在进行大鼠运动功能的研究时，该项目的结果很容易应用于小鼠的运动，并对数百种基因敲除小鼠模型的运动有很大的影响。这项工作将生成能够准确量化运动缺陷的技术，以映射到微妙的神经肌肉病变，并构成理论基础，以研究在更复杂的病变（例如坐骨神经损伤或脊髓损伤）中恢复恢复的机制，例如最终适用于转基因大鼠和小鼠。大鼠和小鼠绝大多数是研究和开发脊髓损伤和其他严重，使人损害神经系统的疗法的首选研究模型。目前，不存在将特定神经肌肉损伤与大鼠特定生物力学缺陷联系起来的能力，因此科学界只能对潜在治疗的疗效得出一般性的结论。该项目：（1）将生成能够准确量化与非常特定的神经肌肉损伤相关的生物力学步态缺陷，（2）为理解更复杂的脊髓损伤（如脊髓损伤）的神经力学补偿机制而产生一个理论基础，以及对步态疾病遗传引起的遗传造成遗传损伤的可能性。