Molecular Epidemiology and Virulence of URTD

URTD 的分子流行病学和毒力

基本信息

批准号：
7390268
负责人：
LORI D WENDLAND
金额：
$ 11.08万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7390268
关键词：
Address Animal Model Animals Antibodies Biological Assay Biological Models Biomedical Research Chronic Chronic Disease Clinical Communicable Diseases Critiques Data Development Diagnostic Discipline Disease Disease model Dose Ecological Change Ecology Epidemiology Exhibits Experimental Designs Exposure to Fishes Florida Foundations Funding Genes Genomics Gophers Grant Human Individual Infection Infectious Agent Insect Vectors Investigation Joints Laboratory Animal Medicine Lesion Location Long-Term Effects Maintenance Medicine Methodology Modeling Molecular Molecular Diagnostic Testing Molecular Epidemiology Morbidity - disease rate Mycoplasma NIH Program Announcements Nature Pathogenesis Pathology Pattern Population Program Development Reptiles Research Research Personnel Resources Respiratory Tract Diseases Respiratory Tract Infections Science Scientist Serological Severities Source Technology Testing Training Training Programs Turtles United States National Institutes of Health Universities Upper respiratory tract Veterinarians Veterinary Medicine Virulence Virulent Work Writing anthropogenesis base college design environmental change exposed human population insight life history loris mortality multidisciplinary parent grant parent project programs species difference tool tool development transmission process

项目摘要

DESCRIPTION (provided by applicant): Many newly emerging diseases are caused by zoonotic agents with wildlife reservoirs or have resulted from Iarge-scale ecological changes that increase human exposure to insect vectors as well as to animal and environmental sources of disease. Approximately 80% of the infectious agents that cause disease in humans are shared with various animal hosts. Veterinarians are good candidates to study these emerging diseases, but training programs aimed at disease pathogenesis and epidemiology specifically tailored to wildlife diseases are lacking. The proposed training will provide a multidisciplinary program in infectious diseases, pathogenesis, molecular diagnostic methodologies, pathology, fundamentals of laboratory animal medicine, epidemiology and disease modeling. The model system chosen for the research component, upper respiratory tract disease (URTD) in gopher tortoises, is an excellent example of chronic disease in a nontraditional animal host. URTD represents an intriguing model to study establishment, transmission, maintenance, and consequences of chronic infection within a population with a life history that parallels humans. The proposed study will address the following hypotheses: (1) URTD is caused by a cluster of closely related mycoplasmal species. The specific species involved may vary within tortoise populations, and these species may differ regionally and, (2) Mycoplasmas vary in virulence, and the specific mycoplasma present in a given population will influence the transmission, clinical course and severity of URTD. The hypotheses will be addressed with the following specific aims: (1) To characterize the molecular epidemiology of mycoplasmal species and selected strains obtained from different tortoise populations; (2) To characterize the virulence of mycoplasmal species and selected strains obtained from different tortoise populations; (3) To develop a multi-plex Luminex assay for quantitation of antibodies to mycoplasmal species from tortoises; and (4) To identify genes associated with virulent and avirulent mycoplasmal species and selected strains. The study of a chronic respiratory infection in a natural population will provide a framework for the development of predictive and casual models that may provide valuable insights into the mechanisms by which chronic infections are maintained and transmitted on a population basis.

描述（由申请人提供）：许多新出现的疾病是由野生动物宿主的人畜共患病原体引起的，或者是由大规模生态变化引起的，这些变化增加了人类与昆虫媒介以及动物和环境疾病源的接触。大约 80% 的引起人类疾病的传染源是与各种动物宿主共有的。兽医是研究这些新出现疾病的良好人选，但缺乏专门针对野生动物疾病的疾病发病机制和流行病学培训计划。拟议的培训将提供传染病、发病机制、分子诊断方法、病理学、实验动物医学基础、流行病学和疾病建模等多学科课程。为研究部分选择的模型系统，即地鼠龟的上呼吸道疾病 (URTD)，是非传统动物宿主慢性疾病的一个很好的例子。 URTD 代表了一个有趣的模型，用于研究具有与人类相似生活史的人群中慢性感染的建立、传播、维持和后果。本研究将提出以下假设：(1) URTD 是由一群密切相关的支原体物种引起的。陆龟种群中所涉及的具体物种可能有所不同，并且这些物种可能因地区而异，并且，（2）支原体的毒力有所不同，特定种群中存在的特定支原体将影响URTD的传播、临床病程和严重程度。这些假设将实现以下具体目标：（1）表征支原体物种和从不同陆龟种群获得的选定菌株的分子流行病学特征； (2) 表征支原体种类和从不同龟种群获得的选定菌株的毒力； (3) 开发一种多重 Luminex 检测方法，用于定量陆龟支原体抗体； (4) 鉴定与强毒力和无毒力支原体物种和选定菌株相关的基因。对自然人群中慢性呼吸道感染的研究将为预测和偶然模型的开发提供一个框架，该模型可能为了解慢性感染在人群中维持和传播的机制提供有价值的见解。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Improved enzyme-linked immunosorbent assay to reveal Mycoplasma agassizii exposure: a valuable tool in the management of environmentally sensitive tortoise populations.

改进的酶联免疫吸附测定法可揭示阿加西支原体暴露情况：管理环境敏感的陆龟种群的宝贵工具。

DOI：
10.1128/cvi.00108-07
发表时间：
2007
期刊：
Clinical and vaccine immunology : CVI
影响因子：
0
作者：
Wendland,LoriD;Zacher,LaurieA;Klein,PaulA;Brown,DanielR;Demcovitz,Dina;Littell,Ramon;Brown,MaryB
通讯作者：
Brown,MaryB

Social behavior drives the dynamics of respiratory disease in threatened tortoises.

社会行为推动了受威胁陆龟呼吸道疾病的发展。

DOI：
10.1890/09-1414.1
发表时间：
2010
期刊：
Ecology
影响因子：
4.8
作者：
Wendland,LoriD;Wooding,John;White,CLeAnn;Demcovitz,Dina;Littell,Ramon;Berish,JoanDiemer;Ozgul,Arpat;Oli,MadanK;Klein,PaulA;Christman,MaryC;Brown,MaryB
通讯作者：
Brown,MaryB

Detection of a novel spotted fever group Rickettsia in the gophertortoise tick.

在地鼠蜱中检测到一种新型斑点热病立克次体。

DOI：
10.1603/me11264
发表时间：
2012
期刊：
Journal of medical entomology
影响因子：
2.1
作者：
Zemtsova,GalinaE;Gleim,Elizabeth;Yabsley,MichaelJ;Conner,LMike;Mann,Tom;Brown,MaryD;Wendland,Lori;Levin,MichaelL
通讯作者：
Levin,MichaelL

Mycoplasma agassizii strain variation and distinct host antibody responses explain differences between enzyme-linked immunosorbent assays and Western blot assays.

阿加西支原体菌株变异和不同的宿主抗体反应解释了酶联免疫吸附测定和蛋白质印迹测定之间的差异。