Regulation of p53 Transcription by Viral Oncoproteins & Covalent Modifications

病毒癌蛋白对 p53 转录的调节

基本信息

批准号：
7495515
负责人：
CHENG-MING CHIANG
金额：
$ 29.83万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-13 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7495515
关键词：
Acetylation Adenovirus E1B 19K Protein Adenoviruses Apoptosis Binding Biochemical Biological Assay CDKN1A gene Cell Cycle Arrest Cell Cycle Regulation Cells Chemicals Chromatin Chromatin Modeling Chromatin Structure Class Code Complex Condition DNA Tumor Viruses Degradation Pathway EP300 gene Event Excision Fractionation Gene Activation Gene Targeting Genes Genetic Transcription Genome Growth HPV-High Risk Histone H2A Histones Human Human Activities Human Papillomavirus ISWI In Vitro Knock-out Laboratories Lesion Low risk HPV MG132 Malignant Neoplasms Mediating Modification Molecular Molecular Chaperones Monitor Oncogene Proteins Other Finding Papillomavirus Pathway interactions Play Polyomaviruses Large T Proteins Promoter Regions Proteasome Inhibitor Protein p53 Proteins Rate Recombinants Recruitment Activity Regulation Reporter Genes Reporting Repression Research Personnel Reverse Transcriptase Polymerase Chain Reaction Risk Role Simian virus 40 Small Interfering RNA Stress TP53 gene Testing Transactivation Transfection Tumor Suppressor Proteins Ubiquitin Viral activating transcription factor cofactor drug development gene repression histone acetyltransferase histone deacetylase 6 human UBE3A protein in vivo inhibitor/antagonist multicatalytic endopeptidase complex mutant novel nucleosome assembly protein I oncoprotein p21 pathogen programs protein degradation protein function reconstitution repaired research study transcription factor ubiquitin ligase ultraviolet irradiation

项目摘要

DESCRIPTION (provided by applicant): p53 is a tumor suppressor protein that functions as a cellular genome guardian. Under normal growth conditions, p53 is kept at low levels due to a fast protein turnover rate. When cells are stressed, p53 becomes stabilized and leads to cell cycle arrest. This transient block allows cells to overcome the stress and efficiently repair DMA lesions, if necessary. When the damage is too severe to be restored, p53 induces apoptosis. The dual function of p53 lies in its ability to act as a sequence-specific transcription factor that activates transcription of gene products involved in cell cycle control and apoptosis. Not surprisingly, p53 is a frequent target for DNA tumor virus-encoded oncoproteins that are able to functionally inactivate p53 and block the activation of p53 target gene transcription. For over a decade, this inhibition of p53 transactivation has been attributed to the effect of these viral oncoproteins on p53 protein stability. Recently, we have uncovered a new pathway for human papillomavirus (HPV)-encoded E6 oncoprotein to inactivate the function of chromatin-bound p53 by inhibiting acetylation of p53 and nucleosomal core histones on the p53-targeted human p21 gene. To understand the repression mechanisms employed by HPV E6 and other DNA tumor virus-encoded oncoproteins, we propose two aims. 1) To identify cellular proteins involved in E6-mediated repression of p53 target gene transcription. We found that both p53 and histone acetyltransferase (HAT) p300 are essential for E6-mediated repression of p21 gene transcription. Since p300 autoacetylation is not inhibited by E6, we hypothesize that acetylated p300 may provide a protein code for recruitment of other cellular proteins to further modify the function of p300 and p53, resulting in a condensed chromatin structure on p53 target genes. This hypothesis will be tested by performing in vitro chromatin transcription, HAT assays, DNA/chromatin-binding assays as well as in vivo ChIP, RT-PCR, siRNA and reporter gene assays to follow the recruitment of transcription factors and cofactors to p53-regulated genes. Moreover, we will identify additional cellular proteins involved in this repression pathway by isolating and characterizing E6 cellular complexes. An unbiased biochemical fractionation will also be conducted to identify cellular factors involved in E6 repressor function. 2) To define the repression mechanism used bv other DNA tumor virus-encoded oncoproteins. We will examine whether SV40 and polyomavirus large T-antigens and adenovirus E1B-55K and E1B-19K proteins also employ a similar but non-identical mechanism to repress p53 target gene transcription. Collectively, these studies will establish a general principle of this novel repression mechanism employed by DNA tumor virus- encoded oncoproteins, independently of the proteasome-mediated degradation pathway, and provide a new direction for the development of drug inhibitors to block the propagation of these human pathogens.

描述（由申请人提供）：p53 是一种肿瘤抑制蛋白，具有细胞基因组守护者的功能。在正常生长条件下，由于蛋白质周转速度快，p53 保持在较低水平。当细胞受到应激时，p53 变得稳定并导致细胞周期停滞。这种短暂的阻断使细胞能够克服压力并在必要时有效修复 DMA 损伤。当损伤太严重而无法恢复时，p53 会诱导细胞凋亡。 p53 的双重功能在于其作为序列特异性转录因子的能力，可激活参与细胞周期控制和细胞凋亡的基因产物的转录。毫不奇怪，p53 是 DNA 肿瘤病毒编码的癌蛋白的常见靶标，这些癌蛋白能够功能性地灭活 p53 并阻断 p53 靶基因转录的激活。十多年来，p53 反式激活的这种抑制被归因于这些病毒癌蛋白对 p53 蛋白稳定性的影响。最近，我们发现了人乳头瘤病毒（HPV）编码的 E6 癌蛋白的新途径，通过抑制 p53 和核小体核心组蛋白在 p53 靶向的人 p21 基因上的乙酰化来灭活染色质结合 p53 的功能。为了了解 HPV E6 和其他 DNA 肿瘤病毒编码的癌蛋白所采用的抑制机制，我们提出了两个目标。 1) 鉴定参与 E6 介导的 p53 靶基因转录抑制的细胞蛋白。我们发现 p53 和组蛋白乙酰转移酶 (HAT) p300 对于 E6 介导的 p21 基因转录抑制至关重要。由于p300自乙酰化不被E6抑制，我们推测乙酰化的p300可能提供用于招募其他细胞蛋白的蛋白质代码，以进一步修饰p300和p53的功能，从而在p53靶基因上产生浓缩的染色质结构。该假设将通过体外染色质转录、HAT 测定、DNA/染色质结合测定以及体内 ChIP、RT-PCR、siRNA 和报告基因测定来检验，以跟踪转录因子和辅助因子向 p53 调节的募集情况基因。此外，我们将通过分离和表征 E6 细胞复合物来鉴定参与该抑制途径的其他细胞蛋白。还将进行无偏生化分级分离，以确定参与 E6 阻遏蛋白功能的细胞因子。 2) 定义其他DNA肿瘤病毒编码的癌蛋白所使用的抑制机制。我们将检查 SV40 和多瘤病毒大 T 抗原以及腺病毒 E1B-55K 和 E1B-19K 蛋白是否也采用类似但不相同的机制来抑制 p53 靶基因转录。总的来说，这些研究将建立 DNA 肿瘤病毒编码癌蛋白所采用的这种新型抑制机制的一般原理，独立于蛋白酶体介导的降解途径，并为开发药物抑制剂以阻止这些人类肿瘤的传播提供新方向。病原体。