Genotypic and Phenotypic Analysis of West Nile virus, Yucatan Peninsula of Mexico

墨西哥尤卡坦半岛西尼罗河病毒的基因型和表型分析

• 批准号: 7394960
• 负责人: Bradley J Blitvich
• 金额: $ 16.02万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-10 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7394960
• 关键词: Animals; Antibodies; Area; Attenuated; Base Sequence; Biological Assay; Birds; Canada; Cessation of life; Competence; Condition; Culicidae; Cultured Cells; Data; Destinations; Disease Outbreaks; Disease Outcome; Epidemic; Equine Encephalomyelitis; Equus caballus; Feeding behaviors; Foundations; Future; Genes; Genetic; Genetic Determinism; Genome; Genomics; Genotype; Goals; Grant; Growth; Health; Human; Infection; Investigation; Island; Kinetics; Medical Surveillance; Membrane; Methods; Mexican; Mexico; Molecular Epidemiology; Morphology; Mus; New York; Pathogenesis; Phenotype; Phylogenetic Analysis; RNA; Recording of previous events; Reporting; Research; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Risk Factors; Site; Temperature; Time; United States; Variant; Vertebrates; Virulence; Virus; Virus Diseases; West Nile virus; day; feeding; human disease; human morbidity; in vivo; interest; invertebrate host; migratory bird; mortality; preference; programs; research study; vector; virus genetics

DESCRIPTION (provided by applicant): The initial outbreak of West Nile virus (WNV) in the Western Hemisphere occurred in New York in 1999, and since then the virus has rapidly spread across the United States and Canada, where it has been responsible for significant human morbidity and mortality. In contrast, there have been no human cases of WNV in the Yucatan Peninsula (YP) of Mexico, nor have there been any reports of widespread WNV associated illness in horses or birds in this region, despite evidence of WNV activity. Indeed, we and our Mexican colleagues at the Universidad Autonoma de Yucatan (DAY) in Merida detected antibodies to WNV in horses, birds and other vertebrate animals in the YP. Most pertinent to this proposal, however, are the findings from a serosurvey conducted on Cozumel Island; antibodies to WNV were detected in 75 of 144 (52%) horses, but none of the horses had signs of illness and none had a history of WNV-like illness. Thus, the underlying hypothesis in this proposal is that the dominant WNV variants in the YP are genotypically and phenotypically distinct from the dominant WNV variants in the U.S. In this grant, we and our colleagues at the UAY will obtain WNV isolates in the YP, and define their genotypes and cell culture phenotypes. In specific aim 1, mosquitoes will be trapped at sites in the YP where seropositive vertebrates have previously been identified. Mosquitoes will be assayed for WNV by RT-PCR and virus isolation. These studies will identify likely vectors of WNV in the YP, thereby providing the groundwork for future vector competence and host-feeding behavior studies. In specific aim 2, WNV isolates will be examined by partial nucleotide sequencing and phylogenetic analysis. Dominant WNV variants will be completely sequenced. In specific aim 3, the cell culture phenotypes (i.e. replication kinetics and yields, plaque morphologies and temperature sensitivities) of dominant WNV variants from the YP and U.S. will be compared. These studies will provide the groundwork for future in vivo pathogenesis studies in vertebrate hosts. RELEVANCE: In this proposal, the genotypes and cell culture phenotypes of dominant WNV variants in the YP will be characterized, and likely vectors of WNV in this region will be identified. This information will help determine why there has not been an epidemic of WNV in the YP, and will identify risk factors associated with WNV disease.

描述（由申请人提供）：西尼罗河病毒（WNV）于 1999 年在纽约首次在西半球暴发，此后该病毒迅速在美国和加拿大传播，造成了严重的人员伤亡。人类发病率和死亡率。相比之下，墨西哥尤卡坦半岛（YP）没有出现西尼罗河病毒人类病例，也没有任何关于该地区马或鸟类广泛传播西尼罗河病毒相关疾病的报告，尽管有证据表明存在西尼罗河病毒活动。事实上，我们和梅里达尤卡坦自治大学 (DAY) 的墨西哥同事在 YP 的马、鸟类和其他脊椎动物中检测到了西尼罗河病毒抗体。然而，与该提议最相关的是在科苏梅尔岛进行的血清调查的结果； 144 匹马中，有 75 匹（52%）检测到了西尼罗河病毒抗体，但没有一匹马有患病迹象，也没有一匹马有类似西尼罗河病毒的疾病史。因此，该提案的基本假设是，YP 中的主要 WNV 变种在基因型和表型上与美国的主要 WNV 变种不同。在这笔拨款中，我们和 UAY 的同事将获得 YP 中的 WNV 分离株，并且定义它们的基因型和细胞培养表型。在具体目标 1 中，蚊子将被困在 YP 中先前已发现血清阳性脊椎动物的地点。将通过 RT-PCR 和病毒分离对蚊子进行 WNV 检测。这些研究将确定 YP 中可能的西尼罗河病毒载体，从而为未来的载体能力和宿主摄食行为研究奠定基础。在具体目标 2 中，将通过部分核苷酸测序和系统发育分析来检查西尼罗河病毒分离株。主要的 WNV 变种将被完全测序。在具体目标 3 中，将比较来自 YP 和美国的主要 WNV 变种的细胞培养表型（即复制动力学和产量、噬斑形态和温度敏感性）。这些研究将为未来脊椎动物宿主体内发病机制研究奠定基础。相关性：在本提案中，将描述 YP 中主要 WNV 变种的基因型和细胞培养表型，并鉴定该区域可能的 WNV 载体。这些信息将有助于确定为什么 YP 没有出现西尼罗河病毒流行，并将确定与西尼罗河病毒疾病相关的危险因素。

项目成果

Antibodies to influenza and West Nile viruses in horses in Mexico.

墨西哥马匹体内的流感和西尼罗河病毒抗体。

• DOI: 10.1136/vr.b5586
• 发表时间: 2010
• 期刊: The Veterinary record
• 作者: Loroño-Pino,MA;Farfan-Ale,JA;Garcia-Rejon,JE;Lin,M;Rosado-Paredes,E;Puerto,FI;Bates,A;Root,JJ;Franklin,AB;Sullivan,HJ;Blitvich,BJ
• 通讯作者: Blitvich,BJ

Complete nucleotide sequences of the small and medium RNA genome segments of Kairi virus (family Bunyaviridae).

• DOI: 10.1007/s00705-009-0465-y
• 发表时间: 2009
• 期刊: ARCHIVES OF VIROLOGY
• 影响因子: 2.7
• 作者: Soto, Victor;Dorman, Karin S.;Miller, W. Allen;Farfan-Ale, Jose A.;Lorono-Pino, Maria A.;Garcia-Rejon, Julian E.;Blitvich, Bradley J.
• 通讯作者: Blitvich, Bradley J.

Genomic sequence and phylogenetic analysis of Culex flavivirus, an insect-specific flavivirus, isolated from Culex pipiens (Diptera: Culicidae) in Iowa.

• DOI: 10.1603/033.046.0428
• 发表时间: 2009-07
• 期刊: Journal of medical entomology
• 影响因子: 2.1
• 作者: Blitvich BJ;Lin M;Dorman KS;Soto V;Hovav E;Tucker BJ;Staley M;Platt KB;Bartholomay LC
• 通讯作者: Bartholomay LC

Antibodies to West Nile virus in wild and farmed crocodiles in southeastern Mexico.

墨西哥东南部野生和养殖鳄鱼体内的西尼罗河病毒抗体。

• DOI: 10.7589/2012-11-290
• 发表时间: 2013
• 期刊: Journal of wildlife diseases
• 影响因子: 1.3
• 作者: Machain-Williams,Carlos;Padilla-Paz,SergioE;Weber,Manuel;Cetina-Trejo,Rosa;Juarez-Ordaz,JoséAlfredo;Loroño-Pino,MaríaAlba;Ulloa,Armando;Wang,Chong;Garcia-Rejon,Julián;Blitvich,BradleyJ
• 通讯作者: Blitvich,BradleyJ

Serologic evidence of flavivirus infection in bats in the Yucatan Peninsula of Mexico.

• DOI: 10.7589/2012-12-318
• 发表时间: 2013-07
• 期刊: Journal of wildlife diseases
• 影响因子: 1.3
• 作者: Machain-Williams C;López-Uribe M;Talavera-Aguilar L;Carrillo-Navarrete J;Vera-Escalante L;Puerto-Manzano F;Ulloa A;Farfán-Ale JA;Garcia-Rejon J;Blitvich BJ;Loroño-Pino MA
• 通讯作者: Loroño-Pino MA