Innovative Membrane Protein Technology: Expression Engineering(IMPEE)

创新膜蛋白技术：表达工程（IMPEE）

• 批准号: 7484100
• 负责人: Scott Allan Lesley
• 金额: $ 151.72万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7484100
• 关键词: Baculoviruses; Biological Assay; Cell-Free System; Cells; Classification; Collaborations; Crystallography; Detergents; Development; Effectiveness; Engineering; Flow Cytometry; Gene Expression; Germ; Goals; Hydrophobicity; In Vitro; Insecta; Ion Channel; Label; Lipids; Mammalian Cell; Membrane; Membrane Proteins; Methods; Miniaturization; Molecular Chaperones; Molecular Profiling; Numbers; Process; Production; Proteins; Protocols documentation; Reaction; Relative (related person); Research; Sampling; Solubility; Solutions; Surveys; System; Techniques; Technology; Testing; Toxic effect; Wheat; cell type; cost; improved; innovation; microbial; new technology; novel; protein expression; receptor; yeast protein

Aim 1. Develop and validate novel methods and technologies for the reliable expression of IMPs. Secondary goal is to reduce the cost of IMP expression. a. Cell free expression - conduct a systematic expression survey with a representative number of IMPs and then develop novel technologies to increase the reliability of the system. b. Baculovirus expression - conduct a systematic expression survey with a representative number of IMPs in different insect cell types. Develop novel methods and technologies to reduce the complexity of the processes, increase reliability of the technology using novel flow cytometry technologies, and lower the cost of expression through miniaturization. c. Mammalian expression - conduct a systematic expression survey with a representative number of IMPs in different mammalian cell types and analyze gene expression profiles during over-expression. Develop novel methods and technologies to reduce the complexity of the processes, increase reliability of the technology using novel flow cytometry technologies, and lower the cost of expression through miniaturization.

目标 1. 开发并验证可靠表达 IMP 的新方法和技术。 第二个目标是降低 IMP 表达的成本。 一个。 无细胞表达 - 使用代表性数量的 IMP 进行系统表达调查，然后开发新技术以提高系统的可靠性。 b. 杆状病毒表达 - 对不同昆虫细胞类型中代表性数量的 IMP 进行系统表达调查。开发新的方法和技术，以降低过程的复杂性，使用新型流式细胞术技术提高技术的可靠性，并通过小型化降低表达成本。 c.哺乳动物表达 - 对不同哺乳动物细胞类型中代表性数量的 IMP 进行系统表达调查，并分析过度表达期间的基因表达谱。开发新的方法和技术，以降低过程的复杂性，使用新型流式细胞术技术提高技术的可靠性，并通过小型化降低表达成本。