Facility Core D--Cell Imaging & Analysis

核心设施 D--细胞成像

• 批准号: 7240049
• 负责人: Juliet A GREENWOOD
• 金额: $ 19.59万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7240049
• 关键词: Affinity; Antibodies; Area; Arts; Binding; Biochemical; Biochemistry; Biomedical Research; Cell Separation; Cells; Collaborations; Complex; Confocal Microscopy; Core Facility; D Cells; Development; Diagnostic Procedure; Disease; Electron Microscopy; Equipment; Evaluation; Exposure to; Flow Cytometry; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Fluorescent Probes; Future; Goals; Green Fluorescent Proteins; Histology; Histopathology; Image; Image Analysis; Imagery; Imaging technology; Individual; Libraries; Life; Methods; Microinjections; Microscopic; Microscopy; Molecular; Molecular Biology; Molecular Probes; Monitor; Mus; Optics; Organelles; Pathologist; Population; Positioning Attribute; Procedures; Productivity; Publications; Publishing; Reagent; Research; Research Personnel; Science; Signal Pathway; Specialist; Speed; Standards of Weights and Measures; System; Technology; Time; Tissues; Toxic effect; Training; Transgenic Organisms; Xenobiotics; Yeasts; Zebrafish; antigen binding; base; cellular imaging; cost; detector; experience; innovative technologies; instrument; instrumentation; prevent; single molecule; tissue culture; tool; two-photon

Imaging of tissues and isolated cells is a critical tool for determining the biochemical and molecular mechanisms involved in phenotypic and functional changes in tissues, cells, and subcellular organelles induced by exposure to xenobiotics. Recent advances in imaging, biological chemistry, and molecular biology, have led to an escalation in imaging technologies and use in the biomedical sciences over the last several years. Development of fluorescent probes, green fluorescent protein (GFP), confocal and two-photon optics, and photoelectronic detectors, have allowed the visualization of single molecules, monitoring of molecular interactions in live cells, and enhanced ability to image complex tissues. The complexity, time, and cost required for developing state-of-the-art imaging technologies makes it unfeasible for individual investigators to independently obtain these important tools and approaches for biomedical research. Therefore, the need exists for an organized group of specialists with the expertise in imaging technologies to acquire the instrumentation, master the applications, and assist individual investigators in using the technology to answer specific research questions. The long-term goal of the Cell Imaging and Analysis Facility is to provide access to state-of-the-art instrumentation and expertise in flow cytometry and cell sorting, histopathology, and microscopy. Our objective is to develop fluorescence-based technologies to meet the current and future needs of Center investigators. Our rationale is that access to state-of-the-art fluorescence technologies will allow Center investigators to determine specific mechanisms of xenobiotic action at the cellular level. The motivated and interactive staff of the facility provides expertise in complementary areas of imaging and analysis. Dr. Kerkvliet and Ms. Oughton have over 40 years combined experience in flow cytometry with an outstanding record of research productivity. Dr. Greenwood and Ms. Fraley have recently setup the confocal and widefield microscopy systems, publishing studies resulting from the development of fluorescence recovery after photobleaching and fluorescence resonance energy transfer microscopy. Dr. Lohr is a certified Veterinary Pathologist with important collaborative interactions with Center investigators demonstrated by recent publications and Ms. Fischer has over 20 years of experience in histology, image analysis, and electron microscopy. Lastly, our collaboration with Molecular Probes/lnvitrogen, providing access to reagents and expertise, makes us especially well-positioned to accomplish our objective.

组织和分离细胞的成像是确定生化和分子的关键工具 涉及组织，细胞和亚细胞细胞器的表型和功能变化的机制 暴露于异种生物。成像，生物学和分子的最新进展 生物学，导致成像技术升级，并在生物医学科学中使用 最近几年。荧光探针，绿色荧光蛋白（GFP）的开发，共聚焦和 两光子光学元件和光电检测器允许单分子的可视化， 监测活细胞中的分子相互作用，并增强图像复杂组织的能力。这 开发最新成像技术所需的复杂性，时间和成本使得 个人调查人员独立获取这些重要工具和方法的不可行 生物医学研究。因此，需要有专业知识的有组织的专家组的需求 在成像技术以获取仪器，掌握应用程序并协助个人 研究人员使用该技术回答特定的研究问题。 细胞成像和分析设施的长期目标是提供最先进的访问权限 流式细胞仪和细胞分选，组织病理学和显微镜的仪器和专业知识。我们的 目的是开发基于荧光的技术，以满足中心的当前和未来需求 调查人员。我们的理由是，进入最先进的荧光技术将允许中心 研究人员确定细胞水平上异种生物作用的特定机制。动机 该设施的互动人员提供了成像和分析互补领域的专业知识。博士 Kerkvliet和Oughton女士拥有40多年的流式细胞仪经验 杰出的研究生产力记录。格林伍德博士和弗莱女士最近设立了 共聚焦和广场显微镜系统，发表研究是由 光漂白和荧光共振能量转移显微镜后的荧光恢复。博士 Lohr是一名经过认证的兽医病理学家，与中心进行重要的协作互动 最近出版物和菲舍尔女士证明的调查人员拥有20多年的经验 组织学，图像分析和电子显微镜。最后，我们与分子的合作 探针/lnvitrogen提供了获得试剂和专业知识的访问，使我们特别适合 实现我们的目标。