Endochondral Potential of Trunk Neural Crest Cells

躯干神经嵴细胞的软骨内电位

• 批准号: 7609122
• 负责人: MARTIN I. GARCIA-CASTRO
• 金额: $ 4.86万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609122
• 关键词: Bone and Cartilage Funding; Cartilage; Cephalic; Development; Fibroblast Growth Factor; In Vitro; Molecular; Neural Crest; Neural Crest Cell; Role; Signal Induction; Signal Transduction; Stem cells; Testing; Thinking; Tissues; Vertebrates; bone; cranium; relating to nervous system

Neural crest cells (NCCs) are ectodermally derived stem cells that differentiate into a wealth of derivatives. NCCs from the cranial region of higher vertebrates generate cartilage and bone. Trunk NCCs don't generate bone or cartilage under normal development, but they can be induced to do so in vitro, or when experimentally grafted in specific bone forming territories of the cranium. We have recently shown that the induction of cranial NCCs occurs during gastrulation, much earlier than previously thought (Basch et al. 2006). Additionally we uncover a neural crest induction mechanism independent of mesodermal and neural tissues. These results depart from current dogmas of neural crest induction and provide a new timeframe for NCCs development. The aim of this proposal is to identify the molecular principles governing the chondrogenic differentiation potential of. this critical stem cell for vertebrate development. We propose to challenge the bone and cartilage potential of trunk NCCs through grafting strategies to prove the role of early signals of induction. Then we will test the effects of caudalizing signals (FGF and Wnt) in the chondrogenic differentiation potential of both trunk and cranial NCC.

神经rest细胞（NCC）是外胚层衍生的干细胞，分化为丰富的财富 衍生物。来自较高脊椎动物的颅区域的NCC会产生软骨和骨骼。 TRUNK NCC在正常发育下不会产生骨头或软骨，但它们可以是 诱导在体外或在实验接枝的特定骨形成区域时 颅骨。我们最近表明，颅NCC的诱导发生在 胃肠道，比以前想象的要早得多（Basch等，2006）。另外，我们发现了 神经rest诱导机制与中胚层和神经组织无关。这些结果 脱离当前神经rest诱导的教条，并为NCCS提供新的时间范围 发展。 该提议的目的是确定有关软骨的分子原理 分化潜力。这种关键的干细胞用于脊椎动物发育。我们建议 通过嫁接策略来挑战躯干NCC的骨骼和软骨潜力以证明 早期归纳信号的作用。然后，我们将测试尾能信号的影响（FGF和 Wnt）在躯干和颅内NCC的软骨分化潜力中。