Physiology Core

生理学核心

基本信息

批准号：
7609124
负责人：
THOMAS O CARPENTER
金额：
$ 22.37万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-04-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7609124
关键词：
Agreement Alkaline Phosphatase Animal Model Animals Arts Biochemical Biochemical Markers Biological Assay Bone Density Cells Data Densitometry Development Evaluation Funding Goals Growth Heart Histologic Histology Human Resources In Situ In Vitro Laboratories Measurement Medical Metabolism Methodology Methods Mission Modification Musculoskeletal Musculoskeletal Diseases Numbers Organ Culture Techniques Osteocalcin Peripheral Physiological Physiology Positioning Attribute Postdoctoral Fellow Preparation Productivity Range Research Research Personnel Rodent Serum Services Skeletal system Slide Specimen Staining method Stains Standards of Weights and Measures Students Systems Analysis TdT-Mediated dUTP Nick End Labeling Assay Techniques Time Tissues Training Urine Work X-Ray Computed Tomography bone bone cell bone turnover calcium phosphate computerized experience in vivo interest member research study response tool

项目摘要

The Physiology Core supports comprehensive, state-of-the-art physiological characterization of bone and other musculoskeletal tissues. The Core is comprised of 3 "subunits" which are coordinated by the director, Dr. Thomas Carpenter. The services of the Core are very heavily subscribed, and it is the largest of the three scientific cores. The Histology and Histomorphometry Subunit, which is co-supervised by Drs. Friedlaender and Carpenter, provides high quality histologic and histomorphometric analyses of skeletal tissue. Recent initiatives have included the development of an in situ TUNEL assay, and new immunohistochemical stains in response to the needs of specific investigators. A recent external audit of the subuint by Dr. David Ke, a nationally recognized expert in rodent histomorphometry, concluded that the services being provided are of extremely high quality. Dr. Caren Gundberg, an internationally recognized expert in the field, supervises the Biochemical Markers of Bone Turnover Subunit. This subunit offers assays of bone turnover makers and serum PTH. Assays for media osteocalcin, calcium, phosphate, and alkaline phosphatase, in mineralizing bone cell cultures are new initiatives in the laboratory. The Bone Densitometry Subunit supports measurement of bone density by peripheral quantitative computerized tomography (pQCT) and by dual energy absorptiometry (PlXlmus) through an agreement with the Cell Core. The specific aims of the Core are to: 1) continue to provide the above summarized services to Center members in a coordinated and efficient manner, thereby extending the range and productivity of their work; 2) to offer training in these techniques to Center members and 3) to continue to develop and evaluate new methodologies relevant to the analysis of skeletal tissue in vivo and in vitro.

生理学核心支持全面、最先进的骨骼生理特征和其他肌肉骨骼组织。核心由 3 个“子单元”组成，由导演托马斯·卡彭特博士。 Core 的服务订阅量非常大，是最大的三大科学核心。组织学和组织形态测量亚单位，由 Drs 共同监督。弗里德兰德和 Carpenter 提供骨骼组织的高质量组织学和组织形态计量学分析。最近的举措包括开发原位 TUNEL 检测和新的免疫组织化学染色以满足特定研究人员的需要。最近，Dr. 对子项目进行了一次外部审计。 David Ke 是一位全国公认的啮齿动物组织形态测量学专家，他得出的结论是，这些服务所提供的都是非常高品质的。 Caren Gundberg 博士是该领域国际公认的专家，负责监督生化中心骨转换亚单位的标记。该亚单元提供骨转换标志物和血清的检测甲状旁腺激素。骨矿化中介质骨钙素、钙、磷酸盐和碱性磷酸酶的测定细胞培养是实验室的新举措。骨密度测量子单元支持通过外周定量测量骨密度通过协议进行计算机断层扫描 (pQCT) 和双能量吸收测定法 (PlXlmus) 与细胞核心。核心的具体目标是： 1）继续向中心提供上述总结的服务成员以协调和高效的方式，从而扩大其工作范围和生产力工作; 2) 向中心成员提供这些技术的培训，以及 3) 继续开发和评估与体内和体外骨骼组织分析相关的新方法。