Analytical and Preparative Core

分析和准备核心

• 批准号: 7219730
• 负责人: FREDERICK C. DE BEER
• 金额: $ 21.92万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2011-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219730
• 关键词: Collaborations; Fluorescent Dyes; High Density Lipoproteins; Individual; Isoelectric Focusing; Label; Lipids; Lipoproteins; Plasma; Production; Protein Isoforms; Proteins; Purpose; Quality Control; Radioisotopes; Research; Resources; Services; Standardization; defective adenoviral vector; experience; helper-dependent adenoviral vector; particle; programs

The purpose of Core B is to facilitate research and stimulate collaboration between the individual projects. Specifically, Core B will offer the following services: 1. Lipoprotein isolation and characterization 2. Plasma lipoprotein analysis 3. Plasma lipid analysis 4. Labeling of proteins/lipoproteins with radioisotopes and fluorescent dyes 5. HDL remnant particle purification and characterization 6. Production of replication-defective adenoviral vectors and helper-dependent adenoviral vectors 7. Purification of total SAA and SAA isoforms 8. Isoelectric focusing Centralization of these facilities will permit an efficient use of resources for the Program and experienced technical staff will provide optimal quality control and standardization across Projects.

核心 B 的目的是促进研究并促进各个项目之间的合作。 具体来说，Core B将提供以下服务： 1. 脂蛋白分离和表征 2. 血浆脂蛋白分析 3. 血浆脂质分析 4. 用放射性同位素和荧光染料标记蛋白质/脂蛋白 5. HDL 残余颗粒纯化和表征 6. 复制缺陷型腺病毒载体和辅助依赖性腺病毒载体的生产 7. 总 SAA 和 SAA 亚型的纯化 8.等电聚焦 这些设施的集中化将有助于有效利用该计划的资源和经验丰富的资源 技术人员将提供跨项目的最佳质量控制和标准化。