The Effect of HIV and HAART on Oral Microbial Colonization and Diversityl

HIV 和 HAART 对口腔微生物定植和多样性的影响

• 批准号: 7668036
• 负责人: YIHONG LI
• 金额: $ 24.24万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7668036
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Actinobacillus actinomycetemcomitans; Acute; Affect; Age; Anti-Bacterial Agents; Anti-Retroviral Agents; Antibiotic Therapy; Bacteria; Biological Assay; CD4 Lymphocyte Count; CD4 Positive T Lymphocytes; Candida; Case-Control Studies; Cell Count; Cessation of life; Chronic; Clinical; Combined Modality Therapy; Data; Defensins; Dental caries; Development; Disease; Environment; Gender; Genomics; Gingivitis; Goals; HIV; HIV Infections; HIV Seropositivity; Highly Active Antiretroviral Therapy; Host Defense; Individual; Infection; Lactobacillus; Lactoferrin; Lead; Measures; Methods; Microbe; Molecular; Mouth Diseases; Muramidase; Numbers; Opportunistic Infections; Oral; Oral cavity; Oral health; Patients; Periodontal Diseases; Persons; Polymerase Chain Reaction; Porphyromonas; Predisposition; Proteins; Public Health; Rate; Recombinant DNA; Research; Ribosomal DNA; Saliva; Salivary; Salivary Proteins; Sampling; Secretory Immunoglobulin A; Sequence Analysis; Streptococcus; Streptococcus mutans; Streptococcus sobrinus; System; Testing; Time; Treponema denticola; Viral; Viral Load result; Virus Diseases; antileukoprotease; base; cytokine; denaturing gradient gel electrophoresis; experience; human SLPI protein; insight; killings; microbial; microbial colonization; microbial community; prevent; programs; prospective; salivary proline-rich proteins

HIV/AIDS, killed 2.8 million persons in 2005, continues to be a major public health problem throughout the world. In the U.S. alone, approximately 40,000 persons become infected with HIV each year. Highly active antiretroviral combination therapy (HAART) has significantly reduced the number of AIDS deaths by 70% since 1996, and has changed the clinical profile of HIV infection from a sub-acute lethal to a chronic ambulatory disease. However, HIV-infected patients experience more mucosal opportunistic infections, more dental caries, and severe periodontal diseases, even after HAART, suggesting that compromised oral micro-environment within the oral cavity is a consequence of the viral infection. The long-term goal of this research is to elucidate the association among the innate host defense system, HIV infection, HAART, and oral microbiota. The objectives of the proposed study are to define the difference in oral microbiota between HIV-infected and non-infected individuals and to determine the effect of subsequent HAART on the dynamic changes of the microbial community in the oral cavity. Our central hypothesis is that HIV infection decreases the level of overall microbial colonization and diversity in the oral cavity and that HAART alters the microbiota so compromised. A corollary hypothesis is that the changes in microbial diversity in HIV+ individuals lead to an increase in cariogenic and periodontal bacteria, resulting in an increase in caries and periodontal disease. To test these hypotheses we will measure the colonization of (a) total cultivable microbes; (b) total Candida spp.; (c) total streptococci; and (d) total lactobacilli in saliva of HIV infected and noninfected subjects; examine the microbial diversity using the 16S rDNA-based approach; , and determine the association among specific salivary proteins of known involvement in HIV infection, specific cariogenic and periodontopathogenic bacterial colonization, and oral health assessments. The findings of this study will provide new insight into whether HIV infection causes changes in oral microbiota and salivary protein profiles favoring the development of caries and periodontal diseases and may the development of specific antibiotic therapy to prevent the adverse change in the microbiota and to decrease susceptibility to other infections

艾滋病毒/艾滋病在2005年被杀280万人，在整个过程中仍然是一个重大的公共卫生问题 世界。仅在美国，每年大约有40,000人感染HIV。 高活性的抗逆转录病毒联合疗法（HAART）显着减少了 自1996年以来，AIDS死亡人数增加了70％，并改变了亚急性的HIV感染的临床特征 致命的慢性门诊疾病。但是，感染HIV的患者会出现更多的粘膜 机会主义感染，更多的龋齿和严重的牙周疾病，即使在Haart之后， 表明口腔内的口服微环境受到损害是由于 病毒感染。这项研究的长期目标是阐明先天的关联 宿主防御系统，HIV感染，HAART和口服微生物群。拟议研究的目标 定义HIV感染和未感染个体之间口服微生物群的差异，以及 确定随后的HAART对微生物群落动态变化的影响 在口腔中。我们的中心假设是HIV感染降低了整体微生物的水平 口腔中的定植和多样性，而Haart则改变了微生物群。一个 推论假设是，艾滋病毒+个体微生物多样性的变化导致增加 在牙科和牙周细菌中，导致龋齿和牙周疾病的增加。 为了检验这些假设，我们将测量（a）总可栽培微生物的定殖； （b）总数 念珠菌属； （c）总链球菌； （d）感染艾滋病毒和未感染的艾滋病毒的唾液中乳酸的总乳酸菌 受试者；使用基于16S rDNA的方法检查微生物多样性； ，确定 已知参与HIV感染的特定唾液蛋白之间的关联，特异性致癌 以及牙周病生成细菌定殖和口腔健康评估。这个发现 研究将提供有关HIV感染是否导致口腔微生物群和 唾液蛋白特征有利于龋齿和牙周疾病的发展，可能 开发特定的抗生素疗法，以防止微生物群的不良变化和 减少对其他感染的敏感性