Core--Quantitative Molecular Assay

核心--定量分子检测

基本信息

批准号：
7284600
负责人：
Kenneth Hadlock
金额：
$ 13.96万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7284600
关键词：
Actins Air Aliquot All Sites Alzheimer&apos s Disease Amyotrophic Lateral Sclerosis Anabolism Animal Model Appendix Biological Assay Buffers Cell Count Cell Line Cell Separation Cells Centrifugation Classification Clinical Complementary DNA Computer software Cost Savings DNA DNA copy number Data Deoxyribonuclease I Deoxyribonucleases Detection Diagnostic Diploidy Disease Drug Delivery Systems Edetic Acid Elevation Endopeptidase K Endoribonucleases Ensure Ethanol FCGR3B gene Fostering Genes Genome Genomics Glycogen Guanidinium Chloride HIV Heating Human Hydroxymethylbilane Synthase Ice Incubated Individual Inflammation Instruction Investigation Label Location Macaca mulatta Manufacturer Name Manuscripts Measurement Methods Molecular Monitor Neurodegenerative Disorders Nucleic Acids Numbers Pancreatic ribonuclease Pathogenesis Patients Performance Phase I Clinical Trials Plasma Polyamines Polymerase Chain Reaction Precipitation Preparation Program Research Project Grants Protocols documentation RNA RNA-Directed DNA Polymerase Reaction Reagent Recommendation Reproducibility Research Project Grants Research Proposals Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Rhodamine Rhodamines Ribonucleases Role Running SIV SYBR Green I Sampling Scheme Score Services Signal Transduction Standards of Weights and Measures Sum Supervision Technology Time Tissues Tube Variant Virion Voting Water Weight austin doxorubicin/etoposide/methotrexate protocol guanidine thiocyanate indexing inhibitor/antagonist interest macrophage melting monocyte nervous system disorder present value prognostic programs receptor uracil-DNA glycosylase

项目摘要

In HIV associated neurological disease (HAND), HIV infected macrophages- and Fcgamma receptor III (CD16) positive macrophages and monocytes in particular, have been associated with disease pathogenesis. However, the differences between normal CD16+ monocytes that appear to have a role in replenishing tissue macrophages and dysfunctional CD16+ monocytes that appear to foster inflammation are not well defined. Pathologica LLC has recently developed a very sensitive molecular assay for the detection of the dysfunctional macrophage subset associated with severe neurodegenerative disease and by inference HIV associated neurological disease. In this program project grant all of the research proposals will make extensive use of this assay in the course of their investigations. Accordingly performance of this multi-gene real time quantitative reverse-transcription-PCR assay will be centralized at Pathologica during the course of this program project. Additionally, Pathologica will provide quantitative HIV and SIV PCR assays for all sites, including HIV proviral load, SIV proviral load, and SIV RNA. These assays will allow for an unambiguous determination if treatment with polyamine biosynthesis inhibitors significantly impacts HIV or SIV replication. The performance of these HIV / SIV assays at one location will help ensure the reproducibility and quality of the assay data for all research projects, as well as results in some cost savings.

在 HIV 相关神经系统疾病 (HAND) 中，HIV 感染的巨噬细胞和 Fcgamma 受体 III (CD16) 阳性巨噬细胞和单核细胞尤其与疾病有关发病。然而，正常 CD16+ 单核细胞之间的差异似乎在补充组织巨噬细胞和功能失调的 CD16+ 单核细胞似乎会促进炎症没有明确定义。 Pathologica LLC 最近开发了一种非常灵敏的分子检测方法来检测与严重神经退行性疾病相关的功能失调的巨噬细胞亚群并由此推断 HIV相关的神经系统疾病。在这个项目中，所有的研究提案都将得到资助在他们的研究过程中广泛使用了这种测定法。因此，这种多基因的表现实时定量逆转录 PCR 检测将在 Pathologica 中心进行本计划项目。此外，Pathologica 将为所有患者提供定量 HIV 和 SIV PCR 检测。位点，包括 HIV 前病毒载量、SIV 前病毒载量和 SIV RNA。这些测定将允许明确确定多胺生物合成抑制剂治疗是否显着影响 HIV 或 SIV 复制。在一个地点进行这些 HIV/SIV 检测将有助于确保所有研究项目的检测数据的可重复性和质量，以及节省一些成本。