Response of Genital Tract HIV-1 to HAART: Role of HSV-2?

生殖道 HIV-1 对 HAART 的反应：HSV-2 的作用？

• 批准号: 7432616
• 负责人: CAROLYN WESTER
• 金额: $ 13.41万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7432616
• 关键词: AIDS prevention; African; Anti-Retroviral Agents; Blood; Botswana; Caring; Cells; Clinic; Cohort Studies; Communicable Diseases; Conduct Clinical Trials; DNA; Disease; Enrollment; Ensure; Epidemic; Genital system; Goals; HIV Infections; HIV-1; Highly Active Antiretroviral Therapy; Human Herpesvirus 2; Individual; Infection; Plasma; Play; RNA; Recommendation; Risk; Role; Screening procedure; Seroprevalences; Specimen; Testing; Ulcer; Woman; Women' s Health; antiretroviral therapy; cohort; design; genital herpes; prospective; response; transmission process

DESCRIPTION (provided by applicant): The applicant's long-term goals are to conduct clinical trials related to HIV prevention, care, and treatment in the African context, particularly with respect to women's health. Genital ulcer disease (GUD) plays an important role in fueling the HIV-1 epidemic. Herpes simplex virus type 2 (HSV-2) is an increasingly important cause of GUD around the world and has been associated with increased HIV-1 genital tract shedding and increased risk of HIV-1 acquisition and transmission. It is not known whether HSV-2 influences HIV-1 genital tract shedding even in the context of highly active antiretroviral therapy (HAART). This information is needed to determine factors influencing HIV-1 infectiousness among women on HAART as well as to formulate recommendations regarding genital herpes suppression among co-infected individuals on HAART. This proposed prospective observational cohort study will test the hypothesis that the effect of HAART on decreasing the presence and quantity of genital tract HIV-1 DMA is reduced by the presence of HSV-2 disease and/or genital tract shedding. Specific aims include: (i) Evaluating the association between HSV-2 serostatus and presence and quantity of genital tract cell-associated HIV-1 DNA among women at baseline and at 6 months post-HAART initiation, (ii) Evaluating the association between genital tract HSV-2 DNA and HIV-1 DNA among HSV-2 / HIV-1 coinfected women at baseline and at 6 months post-HAART initiation, and (iii) Determining HSV-2 seroprevalence among women with advanced HIV-infection. The study plans to enroll 400 HIV-infected women preparing to initiate HAART in Botswana (200 HSV-2 seronegative and 200 HSV-2 seropositive). Blood and genital tract specimens will be collected and analyzed at baseline and 6 months after HAART initiation.

描述（由申请人提供）：申请人的长期目标是在非洲背景下进行与预防艾滋病毒，护理和治疗有关的临床试验，尤其是在妇女健康方面。 生殖器溃疡（GUD）在加油HIV-1流行方面起着重要作用。单纯疱疹病毒2型（HSV-2）是世界范围内GUD越来越重要的原因，并且与HIV-1生殖道脱落的增加以及HIV-1获取和传播的风险增加有关。尚不清楚HSV-2即使在高度活跃的抗逆转录病毒疗法（HAART）的背景下，HSV-2是否会影响HIV-1生殖道脱落。需要此信息来确定影响HAART女性HIV-1传染性的因素，并为HAART共同感染的个体抑制生殖器疱疹的建议。这项拟议的前瞻性观察队列研究将检验以下假设：HAART的影响HSV-2疾病和/或生殖道散开会降低HAART对减少生殖道HIV-1 DMA的存在和数量的影响。具体目的包括：（i）评估HSV-2血清表达与生殖道细胞相关的HIV-1 DNA的存在和数量在基线时与HAART启动后6个月的妇女之间的HIV-1 DNA，（II）评估生殖道HSV-2 DNA和HIV-1 DNA之间的相关性，在HSV-2 / HIV-1的启动中（在6个月中）的启动（在6个月中），并在6个月中（在6个月中）（在6个月中）（在6个月中）（在6个月中（以HA）为基础，并在HAS中（以Ha）为基础，并在6个月中（以Ha）为基础。患有晚期HIV感染女性的HSV-2血清阳性。 该研究计划招募400名准备在博茨瓦纳启动Haart的艾滋病毒感染的妇女（200 HSV-2 Seronegative和200 HSV-2血清阳性）。在基线和HAART启动后6个月后，将收集和分析血液和生殖道标本。