Role of a Cell Wall-Associated Kinase in Zinc Sensing

细胞壁相关激酶在锌传感中的作用

• 批准号: 7568887
• 负责人: Zheng-Hui HE
• 金额: $ 19.28万
• 依托单位: SAN FRANCISCO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7568887
• 关键词: Affect; Animals; Arabidopsis; Biochemical Genetics; Bioinformatics; Blood; Cell Wall; Cell membrane; Cells; Communication; Condition; Data; Depth; Diet; Dietary Zinc; Dissection; Edible Plants; Environment; Extracellular Matrix; Family; Family member; Foundations; Genes; Genetic; Genetic Screening; Goals; Growth; Health; Human; Knock-out; Knowledge; Left; Link; Localized; Mediating; Membrane; Micronutrients; Minerals; Molecular; Molecular Genetics; Monitor; Mouse-ear Cress; Mutation; Organ; Organism; Phosphotransferases; Plant Model; Plant Roots; Plants; Play; Production; Public Health; Publishing; Regulation; Reproduction; Research; Role; Screening procedure; Signal Pathway; Signal Transduction; Solid; Source; Testing; Tissues; Transgenic Organisms; Work; Wound Healing; Zinc; Zinc deficiency; base; experience; extracellular; improved; innovation; loss of function; member; mutant; nutrition; receptor; response; zinc-binding protein

multicellular organisms sense zinc and consequently regulate zinc transport is largely unknown. The main goal of our research is to understand how a family of cell wall-associated receptor kinases (WAK) and WAK-like (WAKL) kinases function in cell-cell and cell-environment communications. We have discovered that WAKL4, a WAK/WAKL family member, plays a critical role in Arabidopsis zinc sensing. Our objective in this application is to elucidate the mechanisms of how plants use WAKL4 to monitor extracellular zinc conditions and to regulate zinc transport. Our central hypothesis is that WAKL4, as a receptor kinase, is one of the key regulatory components in zinc signaling. We propose to test this hypothesis to identify key regulators in Arabidopsis zinc signaling. In Aim 1, we will test if zinc-induced tissue-specific WAKL4 expression plays a role in zinc sensing and zinc transport by identifying the actual components that control zinc-induced WAKL4 expression. A combination of biochemical, genetic and bioinformatic approaches will be used to identify and to determine the roles of the regulatory components. In Aim 2, we will use suppressor screening to identity other critical genes in the WAKL4- mediated zinc signaling. The genetic screening is based on the fact that in the absence of WAKL4, zinc transporter genes fail to respond to external zinc depletion. We will identify mutants whose zinc transporter genes, in absence of WAKL4, remain responding to zinc deficiency. WAKL4 is the first receptor kinase suggested as having a role in mineral sensing and regulation. Our proposed studies are expected to provide critical information in understanding how Arabidopsis uses receptor kinases to sense and respond to zinc, and to significantly advance the understanding of how WAK/WAKL members communicate between the extracellular and intracellular compartments. Relevance to Public Health: Zinc is an essential micronutrient for all organisms and its deficiency is a well-known human-health problem. Our contribution here is expected to be a detailed understanding of how an extracellular matrix-linked receptor kinase plays a role in plant zinc sensing and transport. This contribution is expected to provide the knowledge needed to significantly improve edible plants as better dietary zinc sources for both animals and humans.

多细胞生物感觉锌并因此调节锌转运是未知的。这 我们研究的主要目标是了解一个与细胞壁相关受体激酶（WAK）的家庭如何 和Wak样（WAKL）激酶在细胞 - 环境和环境通信中起作用。我们有 发现WAKL4是WAK/WAKL家庭成员，在拟南芥锌感测中起着至关重要的作用。 我们在此应用中的目标是阐明植物如何使用WAKL4监测的​​机制 细胞外锌条件并调节锌转运。我们的中心假设是Wakl4作为 受体激酶是锌信号传导中的关键调节成分之一。我们建议对此进行测试 假设鉴定拟南芥锌信号传导中的关键调节剂。在AIM 1中，我们将测试锌引起的 组织特异性WAKL4表达通过识别实际 控制锌诱导的WAKL4表达的组件。生化，遗传和 生物信息学方法将用于识别和确定调节的作用 成分。在AIM 2中，我们将使用抑制剂筛选对WAKL4-中的其他关键基因进行身份。 介导的锌信号。遗传筛查是基于以下事实：在没有WAKL4的情况下 转运蛋白基因无法响应外部锌消耗。我们将确定其锌的突变体 在没有WAKL4的情况下，转运蛋白基因仍对锌缺乏症反应。 wakl4是第一个 受体激酶表明在矿物传感和调节中起作用。我们提出的研究是 期望提供关键信息，以了解拟南芥如何使用受体激酶来感知 并回应锌，并大大提高对WAK/WAKL成员的理解 在细胞外和细胞内室之间进行通信。 与公共卫生相关：锌是所有生物的必不可少的微量营养素，其不足是一种 众所周知的人类健康问题。我们在这里的贡献预计将是对 细胞外基质连接受体激酶如何在植物锌感测和转运中起作用。这 预计贡献将提供显着改善可食用植物所需的知识 动物和人类的饮食锌来源。