Tissue Arraying, Immunostaining, and Imaging System

组织阵列、免疫染色和成像系统

• 批准号: 7388654
• 负责人: ANDRES J KLEIN-SZANTO
• 金额: $ 26.35万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388654
• 关键词: Animal Experimentation; Biochemical; Biological Markers; Cancer Biology; Cancer Center; Cells; Center Core Grants; Chemoprevention; Chemotherapy-Oncologic Procedure; Collaborations; Colorectal Cancer; Computer Assisted; Consent; Core Facility; DNA Methylation; Data; Data Analyses; Drug or chemical Tissue Distribution; Equipment; Facility Construction Funding Category; Fox Chase Cancer Center; Histology; Histopathology; Histopathology Facility; Human; Image; Image Analysis; Immunohistochemistry; Laboratories; Malignant Neoplasms; Malignant neoplasm of kidney; Modeling; Molecular; Numbers; Operative Surgical Procedures; Organ; P-30 composite resin; Paraffin Embedding; Pathologist; Pathology; Prevention program; Principal Investigator; Process; Prostate; Reaction; Renal carcinoma; Research Personnel; Research Project Grants; Resources; Role; Sampling; Scoring Method; Services; Site; Specimen; Staining method; Stains; System; Tissue Microarray; Tissues; Tumor Bank; Update; Validation; Work; Zinc; cancer prevention; carcinogenesis; cellular imaging; chemotherapy; experience; human tissue; interest; malignant breast neoplasm; malignant tongue neoplasm; neoplastic; ranpirnase

DESCRIPTION (provided by applicant): A system for tissue arraying, automatic immunostaining and cell/tissue image analysis is requested to enhance two existing Core Facilities at Fox Chase Cancer Center (FCCC). The requested equipment will permit a seamless provision of services including construction of tissue microarrays (TMA), automatic immunostaining of TMA and regular histological sections as well as quantitation of the immunohistochemical reactions using a computer-assisted image analyzer. This system will be managed by the Tumor Bank Facility and the Histopathology Facility supported by Cancer Center Core Grant (P-30). The Core facilities are directed by experienced pathologists who share equipment and laboratory space to enhance the efficiency of the services. The Tumor Bank has accrued over 12,000 specimens of paraffin embedded neoplastic and non-neoplastic human tissues from consented surgical cases from 16 organ sites since its inception in 1990. These tissues provide precious resources for molecular, biochemical and cellular studies for the cancer biology, carcinogenesis, targeted chemotherapy and cancer prevention programs. The Histopathology Facility provides histopathology and immunohistochemistry services to investigators using experimental animal and human models. These two facilities work in close collaboration and synergize to provide the best possible pathology support to FCCC investigators. The requested shared equipment will update and enhance both pathology-associated facilities used by more than 30 Principal Investigators. The requested Advanced Tissue Arrayer will permit an efficient multi-user distribution of tissues from the same specimen, providing the exact type of histology/pathology of interest. Dako Autostainer will expedite the immunohistochemical staining process for a large number of samples. The staining results will then be analyzed by the Automated Cellular Image System III, to achieve precise, quantitative analyses of the data, avoiding the current subjective, semi-quantitative scoring method. This proposal will benefit numerous ongoing and ready to be developed projects at FCCC. Data generated from all these research projects will provide useful information in chemoprevention of breast cancer and tongue cancer, colorectal cancer chemotherapy, role of zinc in prostate carcinogenesis, DNA methylation in renal cancers, and validation of various putative cancer biomarkers.

描述（由申请人提供）：要求组织阵列，自动免疫染色和细胞/组织图像分析的系统，以增强FOX CHASE癌症中心（FCCC）的两个现有核心设施。所需的设备将允许无缝提供服务，包括构建组织微阵列（TMA），自动免疫染色，TMA和常规组织学切片以及使用计算机辅助的图像分析仪对免疫组织化学反应进行定量。该系统将由肿瘤库设施和由癌症中心核心赠款（P-30）支持的组织病理学设施管理。核心设施由经验丰富的病理学家指导，他们共享设备和实验室空间以提高服务效率。自1990年成立以来，肿瘤库从16个器官的同意手术病例中获得了12,000多个石蜡嵌入的肿瘤和非神经塑性人体组织。这些组织为癌症生物学，致癌性，靶向化学治疗和癌症的生物学研究提供了分子，生物化学和细胞研究的宝贵资源。组织病理学设施使用实验动物和人类模型为研究人员提供组织病理学和免疫组织化学服务。这两个设施密切合作，并协同为FCCC调查人员提供最佳的病理支持。所请求的共享设备将更新和增强30多个主要研究人员使用的病理相关设施。请求的晚期组织阵列将允许从同一标本中有效的组织分布，提供目的的组织学/病理学的确切类型。 Dako Autostainer将加快大量样品的免疫组织化学染色过程。然后，将通过自动细胞图像系统III分析染色结果，以实现数据的精确定量分析，以避免当前的主观，半定量评分方法。该提案将使FCCC的众多持续不断的项目受益。从所有这些研究项目产生的数据将提供有用的信息，可提供有关乳腺癌和舌癌的化学预防，结直肠癌化学疗法，锌在前列腺癌发生，DNA甲基化在肾癌中的作用以及对各种推定的癌症生物标志物的验证。