ATOMIC FORCE MICROSCOPE

原子力显微镜

• 批准号: 7394176
• 负责人: Mark Paul Goldberg
• 金额: $ 27.5万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7394176
• 关键词: Alzheimer' s Disease; Atomic Force Microscopy; Bacteriology; Biomedical Engineering; Brain Neoplasms; Chemistry; Condition; Contract Services; Core Facility; Data Analyses; Equipment; Financial Support; Fluorescence Microscopy; Funding; Funding Applicant; Future; Grant; Head; Human Resources; Microfluidics; Microscope; Microscopy; Minor; Molecular Analysis; Nanotechnology; Nerve Degeneration; Neurology; Optics; Parkinson Disease; Publishing; Research; Research Personnel; Scanning; Scanning Probe Microscopes; Schedule; Technical Expertise; Training; Tumor Biology; United States National Institutes of Health; Universities; Update; Washington; Yin; cell motility; cellular imaging; digital; experience; fluorescence imaging; instrument; instrumentation; medical schools; nervous system disorder; protein aggregation; protocol development; vibration

DESCRIPTION (provided by applicant): Funds are requested to establish an integrated Atomic Force Microscopy (AFM) facility for NIH-funded research at Washington University School of Medicine. There is a pressing need for updated AFM capabilities as there is currently no AFM on the School of Medicine campus, and AFM instruments elsewhere at Washington University are fully utilized and technically outdated. The proposal will support 22 NIH grant projects of 6 Major Users (with published AFM experience), and 7 Minor Users. Planned AFM applications include cell motility, bacteriology, brain tumor biology, microfluidics, and nanotechnology. Because an important research focus is protein aggregation in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases, the AFM will be acquired by the Hope Center for Neurological Disorders, an interdepartmental research unit administered within the Department of Neurology. The Hope Center will provide financial support for renovation, service contracts, and personnel. Installation within an established microscopy core facility will assist in user training, protocol development, scheduling, data analysis and dissemination. A highly experienced AFM technician will train and assist users. Strong technical expertise for the AFM facility is provided by co-investigators Frank Yin (Department of Biomedical Engineering) and Karen Wooley (Department of Chemistry), major users who operate current Washington University AFM labs. The requested instrumentation includes a Veeco (formerly Digital) Nanoscope V control station operating two scanning probe heads: PicoForce MultiMode (optimized for atomic scale molecular analysis), and BioScope II (for cellular imaging and simultaneous AFM/fluorescence imaging on a Zeiss Axiovert optical microscope), together with required equipment for vibration isolation and environmental control. The proposed configuration will allow optimal integration of optical microscopy and other AFM facilities at Washington University, with potential for significant future expansion as user needs increase.

描述（由申请人提供）：要求资金在华盛顿大学医学院建立综合的原子力显微镜（AFM）设施，用于NIH资助的研究。迫切需要更新的AFM功能，因为医学院校园目前没有AFM，而华盛顿大学其他地方的AFM仪器已充分利用和技术过时。该提案将支持6个主要用户（具有AFM经验）和7个次要用户的22个NIH赠款项目。计划的AFM应用包括细胞运动，细菌学，脑肿瘤生物学，微流体和纳米技术。由于重要的研究重点是在神经退行性疾病（例如阿尔茨海默氏症和帕金森氏病）中的蛋白质聚集，因此AFM将被Hope神经系统疾病中心（一个神经学部门跨部门研究部门）获取。希望中心将为翻新，服务合同和人员提供财政支持。在已建立的显微镜核心设施中安装将有助于用户培训，协议开发，调度，数据分析和传播。经验丰富的AFM技术人员将培训和协助用户。 AFM设施的强大技术专业知识由共同投资者Frank Yin（生物医学工程系）和Karen Wooley（化学系），他们经营现任华盛顿大学AFM Labs的主要用户。 请求的仪器包括一个VEECO（以前是数字）纳米镜v控制站操作两个扫描探针头：Picoforce多模型（针对原子量表分子分析进行了优化）和Bioscope II（用于在Zeiss axioVertical axioVertical Intival Offirention上的蜂窝成像和同时成像和同时的AFM/荧光成像，以及所需的设备。所提出的配置将允许华盛顿大学的光学显微镜和其他AFM设施的最佳整合，并且随着用户需求的增加而有可能发生重大扩展。