A Novel Mechanism for Intra-articular Nonunion

关节内骨不连的新机制

• 批准号: 7495007
• 负责人: Martha M Murray
• 金额: $ 35.61万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-07 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7495007
• 关键词: Algorithms; Anterior Cruciate Ligament; Area; Arthritis; Articular ligaments; Autologous; Bilateral; Biological Assay; Biology; Biomechanics; Blood Platelets; Blood Vessels; Cartilage; Cell Count; Cell Proliferation; Cells; Cicatrix; Clinical; Coagulation Process; Collagen; Dose; Environment; Failure; Family suidae; Fibrin; Healed; Hemorrhage; Histologic; Histology; Human; Hydrogels; Implant; In Vitro; Injury; Invaded; Invasive; Joints; Knee; Left; Length; Ligaments; Magnetic Resonance Imaging; Measures; Mechanics; Meniscus structure of joint; Modeling; Noise; Numbers; Outcome; Outcome Measure; Paired Comparison; Patients; Plug-in; Process; Property; Rate; Readability; Role; Signal Transduction; Site; Stress; Structure of medial collateral ligament of knee joint; Surgical sutures; Synovial Fluid; Tendon structure; Time; Tissue Model; Tissues; Validation; Week; Work; Wound Healing; base; functional outcomes; healing; implantation; improved; in vivo; injury and repair; migration; novel; repaired; research study; response; scaffold; wound

DESCRIPTION (provided by applicant): Injuries to intra-articular tissues, including the anterior cruciate ligament (ACL), menisci, cartilage and tendon, are of great clinical importance. Over 1 million patients each year sustain these injuries and the high rate of failure of repair of these injuries (as high as 90%) leads to loss of tissue and joint integrity and high rates of premature arthritis. Our preliminary work has demonstrated that a collagen-platelet hydrogel can stimulate healing in the wound site of a complete ACL transection. In the work outlined in this proposal, we wish to further understand the mechanisms behind the ability of platelets to stimulate wound healing in the joint in vivo. In our first aim, we propose to study the influence of platelet concentration on the cellular and vascular repopulation of the ACL wound site using in vitro and in vivo assays. The second aim focuses on defining the effects of differences in wound site biology with the return of strength to the ligament as a functional outcome measure. As a secondary outcome, correlations between the biomechanical properties and findings on magnetic resonance imaging (volume measures and signal to noise ratios) will also be sought to enable us to begin to identify non-invasive measure for functional healing of this important intra-articular ligament.

描述（由申请人提供）：关节内组织的伤害，包括前交叉韧带（ACL），半月板，软骨和肌腱，具有极大的临床重要性。每年有超过100万患者维持这些伤害，这些伤害的修复率很高（高达90％）会导致组织和关节完整性的丧失以及高度关节炎的高率。我们的初步工作表明，胶原蛋白 - 斑点水凝胶可以刺激完整ACL横断的伤口部位的愈合。在该提案中概述的工作中，我们希望进一步了解血小板刺激体内关节伤口愈合能力背后的机制。在我们的第一个目的中，我们建议使用体外和体内测定法研究血小板浓度对ACL伤口部位的细胞和血管再生的影响。第二个目的是定义伤口部位生物学差异的影响，而强度恢复为韧带作为功能结果指标。作为次要结果，也将寻求生物力学特性与磁共振成像的发现之间的相关性（体积测量和信号与噪声比），也将寻求使我们能够开始识别这种重要的内膜内韧带的功能愈合的非侵入性措施。