Renal Tissue Derived From Amniotic Fluid Stem Cells

羊水干细胞衍生的肾组织

基本信息

批准号：
7478349
负责人：
Roger Edward De Filippo
金额：
$ 11.74万
依托单位：
CHILDREN'S HOSPITAL OF LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-30 至 2010-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7478349
关键词：
Adult American Amniotic Fluid Anatomy Animal Model Biology Biomedical Engineering Cell Line Cells Coculture Techniques Data Development Developmental Biology ES Cell Line Embryo Embryonic Development Embryonic Structures End stage renal failure Epidemic Goals Growth Factor Histologic In Vitro Individual Kidney Kidney Diseases Kidney Failure Kidney Transplantation Laboratories Lead Mesenchyme Metanephric Diverticulum Metanephric structure Mus Natural regeneration Nude Mice Organ Organ Donor Pathway interactions Patients Population Process Property Renal Tissue Research Personnel Retrieval Role Signaling Protein Source Staging Stem cells System Technology Testing Therapeutic Time Tissue Engineering Tissues Transplantation United States amniotic fluid derived stem cell concept embryo tissue in vivo insight interest kidney cell mutant mouse model novel novel strategies programs scale up tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): A growing shortage of donor organs has heightened interest in developing novel strategies for tissue regeneration, including the possible role of stem cells, as an alternative to heterologous organ replacement in patients with renal failure. We have discovered that a unique population of pluripotential cells exist in amniotic fluid that have similar totipotent properties as more traditional lines of embryonic stem cells but avoid embryo destruction during retrieval, which is desirable. We would like to apply these amniotic fluid derived stem cells for the regeneration of kidney tissue in the laboratory. Tissue Engineering and Developmental Biology have uniquely contributed compelling but preliminary data towards a common goal of bioengineering kidneys. Therefore, the broad objective of this proposal is to determine if amniotic fluid derived stem cells are capable of forming kidney tissue in a novel co-culture system that employs, for the first time, technologies from both Tissue Engineering and Developemental Biology. This project also seeks to optimize the scalability of these bioengineered kidneys so that they can be applied in vivo.

描述（由申请人提供）：越来越多的供体器官的短缺对制定新型组织再生策略（包括干细胞的可能作用）提高了兴趣，这是肾衰竭患者中异源器官替代的一种替代方法。我们已经发现，羊水中存在独特的多能细胞种群，具有与传统的胚胎干细胞系相似的整数特性，但避免在检索过程中避免胚胎破坏，这是值得期望的。我们想将这些羊水衍生的干细胞应用于实验室中肾脏组织的再生。组织工程和发育生物学对生物工程肾脏的共同目标贡献了引人注目但初步的数据。因此，该提案的广泛目标是确定羊水得出的干细胞是否能够在新型的共培养系统中形成肾脏组织，该系统首次采用了组织工程和发展生物学的技术。该项目还旨在优化这些生物工程肾脏的可扩展性，以便可以在体内应用它们。