Development and maintenance of the alveolar type 1 cell

肺泡 1 型细胞的发育和维持

基本信息

批准号：
7386391
负责人：
Tushar Jasubhai DESAI
金额：
$ 13.21万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-01-04 至 2012-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7386391
关键词：
Adult Advisory Committees Alveolar Alveolar Cell Alveolus Antibodies Area Biochemistry Biological Markers Boston Cell Cycle Kinetics Cell Differentiation process Cells Cellular biology Chairperson Critical Care Cultured Cells Databases Defect Development Differentiation Antigens Disease Distal Embryo Embryonic Development Ensure Epithelial Cells Epithelium Event Fellowship Funding Gases Gene Expression Genes Genetic Genetic Recombination Genetic Techniques Genomics Goals Health Histology In Situ Hybridization Indirect Immunofluorescence Individual Institutes Label Laboratories Lung Lung diseases Maintenance Malignant neoplasm of lung Medical Medicine Mentors Molecular Molecular Biology Molecular Genetics Morphogenesis Mouse Strains Mus Natural regeneration Pathway interactions Pattern Phenotype Physicians Physiology Play Pregnancy Principal Investigator Program Development Protein C Protocols documentation Pulmonary Emphysema Pulmonary Fibrosis Pulmonology RNA Research Research Personnel Resources Respiratory Failure Role Scientist Series Signal Transduction Site Specific qualifier value Staging Stem cells Techniques Testing Time Tissues Training Training Programs Transcription factor genes Transgenic Mice Undifferentiated Universities Work abstracting authority base career cell type day in vivo instructor lung Carcinoma lung development mouse genome professor progenitor programs recombinase research study selective expression surfactant transcription factor

项目摘要

DESCRIPTION (provided by applicant): This new investigator proposal describes a 5 year training program for the development of a career as a Physician scientist in pulmonary medicine. The principal investigator has completed a fellowship in pulmonary and critical care medicine at Boston University and a one year research fellowship in the Department of Biochemistry at Stanford University, where he now continues as an Instructor in the Pulmonary and Critical Care division of the Department of Medicine. In carrying out the proposed research, the Principal Investigator will acquire expertise in lung histology, mouse genetics, molecular biology, and genomic approaches. Dr. Mark A. Krasnow will mentor the Principal Investigator's scientific development. He is Professor and Chairman of Biochemistry at Stanford and an Investigator in the Howard Hughes Medical Institute who has trained numerous individuals who have gone on to establish their own laboratories and successfully compete for independent funding. The Principal Investigator has also enlisted three internationally recognized authorities in the fields of lung epithelial cell biology, alveolar development and mouse genetics, respectively, as consultants. He will work closely with the mentor and consultants to ensure fulfillment of the specific aims. An advisory committee of senior investigators at Stanford has also been assembled and will provide scientific and career advice. Research will focus on development and maintenance of the alveolar type 1 (AT 1) cell. Specific aims include: 1) Identify and characterize using molecular markers the putative bi-potential progenitor of AT 1 cells in mouse lung and determine when and where it arises, and how it changes during lung development, 2) Use mouse transgenic techniques to label and trace the fate of mature AT 2 cells to determine whether the AT 2 cell is an obligate progenitor to the AT 1 cell, 3) To identify potential regulators of AT 1 fate by using RNA in situ hybridization to screen for transcription factor genes that are selectively expressed in terminal airspace epithelium at the time of AT 1 morphogenesis. By bringing together unique resources from multiple departments, this program integrates specialized areas of expertise that will ensure fulfillment of the specific aims. This work will be relevant for understanding and ultimately treating lung diseases like emphysema, lung cancer, and pulmonary fibrosis. (End of Abstract)

描述（由申请人提供）：该新的研究者建议描述了一项为期5年的培训计划，以发展职业为肺医学的医师科学家。这位首席研究人员已在波士顿大学完成了肺和重症监护医学奖学金，并在斯坦福大学生物化学系完成了一年的研究奖学金，现在他继续担任医学院肺部和重症监护室的讲师。在进行拟议的研究时，首席研究者将获得肺部组织学，小鼠遗传学，分子生物学和基因组方法方面的专业知识。 Mark A. Krasnow博士将指导主要研究者的科学发展。他是斯坦福大学生物化学的教授兼董事长，也是霍华德·休斯医学院（Howard Hughes Medical Institute）的调查员，他们培训了许多人，他们曾经建立自己的实验室并成功竞争独立资金。首席研究者还分别以顾问为肺上皮细胞生物学，肺泡发育和小鼠遗传学领域的三名国际认可的当局。他将与导师和顾问紧密合作，以确保实现特定目标。斯坦福大学高级调查员的咨询委员会也已经组装，并将提供科学和职业建议。研究将集中于1型牙槽1（1）细胞的开发和维护。 Specific aims include: 1) Identify and characterize using molecular markers the putative bi-potential progenitor of AT 1 cells in mouse lung and determine when and where it arises, and how it changes during lung development, 2) Use mouse transgenic techniques to label and trace the fate of mature AT 2 cells to determine whether the AT 2 cell is an obligate progenitor to the AT 1 cell, 3) To identify potential regulators of AT 1 fate by using RNA in situ与筛选的转录因子基因的杂交基因在1时在末端空域上皮中有选择性表达。通过将来自多个部门的独特资源汇总在一起，该计划集成了专业知识的专业领域，以确保实现特定目标。这项工作将与理解和最终治疗肺气肿，肺癌和肺纤维化等肺部疾病有关。（抽象的结尾）