Development and maintenance of the alveolar type 1 cell

肺泡 1 型细胞的发育和维持

基本信息

批准号：
7386391
负责人：
Tushar Jasubhai DESAI
金额：
$ 13.21万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-01-04 至 2012-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7386391
关键词：
Adult Advisory Committees Alveolar Alveolar Cell Alveolus Antibodies Area Biochemistry Biological Markers Boston Cell Cycle Kinetics Cell Differentiation process Cells Cellular biology Chairperson Critical Care Cultured Cells Databases Defect Development Differentiation Antigens Disease Distal Embryo Embryonic Development Ensure Epithelial Cells Epithelium Event Fellowship Funding Gases Gene Expression Genes Genetic Genetic Recombination Genetic Techniques Genomics Goals Health Histology In Situ Hybridization Indirect Immunofluorescence Individual Institutes Label Laboratories Lung Lung diseases Maintenance Malignant neoplasm of lung Medical Medicine Mentors Molecular Molecular Biology Molecular Genetics Morphogenesis Mouse Strains Mus Natural regeneration Pathway interactions Pattern Phenotype Physicians Physiology Play Pregnancy Principal Investigator Program Development Protein C Protocols documentation Pulmonary Emphysema Pulmonary Fibrosis Pulmonology RNA Research Research Personnel Resources Respiratory Failure Role Scientist Series Signal Transduction Site Specific qualifier value Staging Stem cells Techniques Testing Time Tissues Training Training Programs Transcription factor genes Transgenic Mice Undifferentiated Universities Work abstracting authority base career cell type day in vivo instructor lung Carcinoma lung development mouse genome professor progenitor programs recombinase research study selective expression surfactant transcription factor

项目摘要

DESCRIPTION (provided by applicant): This new investigator proposal describes a 5 year training program for the development of a career as a Physician scientist in pulmonary medicine. The principal investigator has completed a fellowship in pulmonary and critical care medicine at Boston University and a one year research fellowship in the Department of Biochemistry at Stanford University, where he now continues as an Instructor in the Pulmonary and Critical Care division of the Department of Medicine. In carrying out the proposed research, the Principal Investigator will acquire expertise in lung histology, mouse genetics, molecular biology, and genomic approaches. Dr. Mark A. Krasnow will mentor the Principal Investigator's scientific development. He is Professor and Chairman of Biochemistry at Stanford and an Investigator in the Howard Hughes Medical Institute who has trained numerous individuals who have gone on to establish their own laboratories and successfully compete for independent funding. The Principal Investigator has also enlisted three internationally recognized authorities in the fields of lung epithelial cell biology, alveolar development and mouse genetics, respectively, as consultants. He will work closely with the mentor and consultants to ensure fulfillment of the specific aims. An advisory committee of senior investigators at Stanford has also been assembled and will provide scientific and career advice. Research will focus on development and maintenance of the alveolar type 1 (AT 1) cell. Specific aims include: 1) Identify and characterize using molecular markers the putative bi-potential progenitor of AT 1 cells in mouse lung and determine when and where it arises, and how it changes during lung development, 2) Use mouse transgenic techniques to label and trace the fate of mature AT 2 cells to determine whether the AT 2 cell is an obligate progenitor to the AT 1 cell, 3) To identify potential regulators of AT 1 fate by using RNA in situ hybridization to screen for transcription factor genes that are selectively expressed in terminal airspace epithelium at the time of AT 1 morphogenesis. By bringing together unique resources from multiple departments, this program integrates specialized areas of expertise that will ensure fulfillment of the specific aims. This work will be relevant for understanding and ultimately treating lung diseases like emphysema, lung cancer, and pulmonary fibrosis. (End of Abstract)

描述（由申请人提供）：这项新的研究者提案描述了一项为期 5 年的培训计划，旨在发展肺医学医师科学家的职业生涯。主要研究者已在波士顿大学完成了肺科和重症监护医学的研究金，并在斯坦福大学生物化学系完成了为期一年的研究奖学金，目前他继续在斯坦福大学医学系肺科和重症监护科担任讲师。在开展拟议的研究时，首席研究员将获得肺组织学、小鼠遗传学、分子生物学和基因组方法方面的专业知识。 Mark A. Krasnow 博士将指导首席研究员的科学发展。他是斯坦福大学生物化学系的教授兼主席，也是霍华德休斯医学研究所的研究员，他培训了许多个人，这些人后来建立了自己的实验室并成功竞争独立资助。首席研究员还聘请了三位国际公认的肺上皮细胞生物学、肺泡发育和小鼠遗传学领域的权威人士作为顾问。他将与导师和顾问密切合作，以确保实现具体目标。斯坦福大学高级研究人员组成的咨询委员会也已成立，将提供科学和职业建议。研究重点是肺泡 1 型 (AT 1) 细胞的发育和维持。具体目标包括：1) 使用分子标记识别和表征小鼠肺中 AT 1 细胞的假定双电位祖细胞，并确定其出现的时间和地点，以及它在肺发育过程中如何变化，2) 使用小鼠转基因技术来标记和表征追踪成熟 AT 2 细胞的命运，以确定 AT 2 细胞是否是 AT 1 细胞的专性祖细胞，3) 通过使用 RNA 原位杂交筛选来鉴定 AT 1 命运的潜在调节因子用于在 AT 1 形态发生时在终末空腔上皮中选择性表达的转录因子基因。通过汇集多个部门的独特资源，该计划整合了专业领域的专业知识，以确保实现特定目标。这项工作将有助于理解和最终治疗肺气肿、肺癌和肺纤维化等肺部疾病。（摘要完）