Genetics of postdauer developmental timing in C elegans

线虫后发育时间的遗传学

• 批准号: 7547527
• 负责人: XANTHA KARP
• 金额: $ 1.35万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-09 至 2008-05-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7547527
• 关键词: Caenorhabditis elegans; Cell Lineage; Cell division; Condition; Development; Embryonic Development; Environment; Genes; Genetic; Genetic Screening; Goals; Growth; Larva; Malignant Neoplasms; Maps; Mutation; Organism; Pathway interactions; Phenotype; Process; Psyche structure; Regulation; Relative (related person); Signal Transduction; Staging; Techniques; Time; Tissues; Training; Work; genetic analysis; improved; mutant; novel strategies; programs; research study

DESCRIPTION (provided by applicant): The coordinated timing of cell divisions and differentiation between different tissues and between the organism and the environment are critical aspects of normal growth. The control of these processes is important, as cell division irrespective of growth control signals is a hallmark of cancer. Heterochronic genes in C. elegans control the relative timing of cell divisions and differentiation during development. Mutations in these genes result in some cell lineages either omitting or reiterating parts of their developmental program, leading to a variety of abnormal tissues. However, the mutant phenotypes of many of these genes can be suppressed if larvae develop through an alternative pathway called "dauer", chosen in unfavorable conditions. This suggests that some previously characterized heterochronic genes are dispensable for postdauer development, raising the question of what genes control developmental timing in postdauer larvae. The objective of this project is to identify and characterize such genes. Specific Aim 1: To carry out genetic screens to isolate mutants defective in developmental timing postdauer. Specific Aim 2: Genetic analysis of genes isolated in the above screens. Specific Aim 3: To map and clone mutants isolated in the screens.

描述（由申请人提供）：不同组织之间以及生物体与环境之间的细胞分裂和分化的协调时间是正常生长的关键方面。这些过程的控制很重要，因为无论生长控制信号如何，细胞分裂都是癌症的标志。秀丽隐杆线虫中的异时基因控制发育过程中细胞分裂和分化的相对时间。这些基因的突变导致一些细胞谱系省略或重复其发育程序的部分内容，从而导致各种异常组织。然而，如果幼虫通过在不利条件下选择的称为“dauer”的替代途径发育，则许多这些基因的突变表型可以被抑制。这表明一些先前表征的异时基因对于后期发育来说是可有可无的，从而提出了哪些基因控制后期幼虫发育时间的问题。该项目的目标是识别和表征这些基因。具体目标1：进行遗传筛选以分离发育时间缺陷的突变体。具体目标 2：对上述筛选中分离的基因进行遗传分析。具体目标 3：绘制和克隆筛选中分离的突变体。