CORE--Tissue Culture and Immunoassay

核心——组织培养和免疫分析

• 批准号: 7392741
• 负责人: Roberto P Garofalo
• 金额: $ 15.44万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7392741
• 关键词: Arts; Biological; Biological Assay; Child health care; Communication; Core Facility; Cost Savings; Cultured Cells; Epithelial; Human; Human Cell Line; Human Resources; Immunoassay; Laboratories; Location; Maintenance; Medical; Mus; Numbers; Performance; Procedures; Purpose; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Respiratory syncytial virus; Signal Transduction; Standardization; Sucrose; Texas; Tissue Banks; Universities; Viral; airway inflammation; base; chemokine; cost; cytokine; laboratory facility; performance tests; programs; research study; tissue culture; Arts; Biological; Biological Assay; Child health care; Communication; Core Facility; Cost Savings; Cultured Cells; Epithelial; Human; Human Cell Line; Human Resources; Immunoassay; Laboratories; Location; Maintenance; Medical; Mus; Numbers; Performance; Procedures; Purpose; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Respiratory syncytial virus; Signal Transduction; Standardization; Sucrose; Texas; Tissue Banks; Universities; Viral; airway inflammation; base; chemokine; cost; cytokine; laboratory facility; performance tests; programs; research study; tissue culture

This is a proposal for a Tissue Culture and Immunoassay Core (TCIC) facility which purpose is to provide a centralized resource for cell culture maintenance, cellular and viral purification, and immunossay performance, in support of five projects included in the Program Project entitled" Signaling in Airway Inflammation" at University of Texas Medical Branch (UTMB). Specific objectives of the TCIC to provide infrastructure necessary to: 1. Maintain a tissue bank of human cell lines (mostly of epithelial origin); 2. Prepare pools of sucrose-purified respiratory syncytial virus; 3. Perform immunoassays for human and mouse cytokines and chemokines. The support of the TCIC facility is justified based on the critical need for a number of biological reagents, methodological procedures and immunological assays, common to all five projects of the Program Project. In addition, centralized acquisition of reagents and performance of the tests will minimize the cost of M&O and personnel for the whole center and will result in an optimal standardization of assays and experimental procedures. The proposed TCIC facility will take advantage of some resources currently available to the Program Project investigators at UTMB, including the Child Health Research Center (CHRC) laboratory, a 5,000 sq. ft., state-of-art laboratory facility where the TCIC will be physically located. The central location of the TCIC within the UTMB campus, will facilitate the communications among the different investigators of the Program project, resulting in a more efficient and timely completion of the proposed research.

这是组织培养和免疫测定核心（TCIC）设施的一项建议，目的是为维持细胞培养，细胞和病毒纯化以及免疫效果提供集中资源，以支持该计划项目中包含的五个项目，这些项目题为“在德克萨斯大学医学分支机构（UTMB）的“气道炎症信号”（UTMB）。 TCIC的具体目标，以提供以下必要的基础设施：1。 人类细胞系（主要是上皮起源）； 2.准备蔗糖纯化的呼吸道合胞病毒； 3。对人和小鼠细胞因子和趋化因子进行免疫测定。基于对计划项目的所有五个项目共有的许多生物学试剂，方法论程序和免疫学测定的批判需求，TCIC设施的支持是合理的。此外，对试剂的集中获取和测试的性能将使整个中心的M＆O和人员的成本降至最低，并将导致测定和实验程序的最佳标准化。拟议的TCIC设施将利用UTMB计划项目调查人员目前可用的一些资源，包括儿童健康研究中心（CHRC）实验室，该实验室是5,000平方英尺，最先进的实验室实验室设施，该设施将在物理上位于物理上。 TCIC在UTMB校园内的中心位置将促进该计划项目的不同研究人员之间的通信，从而使拟议的研究更加有效，及时完成。