Protein Chemistry Laboratory Core

蛋白质化学实验室核心

基本信息

批准号：
7315557
负责人：
JOHN D LAMBRIS
金额：
$ 23.23万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-01 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7315557
关键词：
Animals Antibodies Architecture Biochemical Bioinformatics Biological Assay Blood C5a anaphylatoxin receptor Cell Fractionation Cell Proliferation Cells Collection Complement Complement 3a Complement Receptor Coupling Disease model Electrophoresis Experimental Models Genes Genetic High Pressure Liquid Chromatography Image Injury Laboratories Liquid Chromatography MALDI-TOF Mass Spectrometry Mass Spectrum Analysis Modeling Molecular Monitor Mus Organelles Pattern Peptides Phosphopeptides Phosphorylation Plasma Preparation Procedures Protein Analysis Protein Chemistry Proteins Proteome Proteomics Purpose Reagent Regulation Resources Serum Serum Proteins Services Signal Transduction Site Specimen Spectrometry Staging Subcellular Fractions Surface Plasmon Resonance System Techniques Technology Time Tissues base cytokine design direct application fast protein liquid chromatography in vivo interest ionization mass spectrometer member neutralizing antibody polyclonal antibody programs protein expression protein protein interaction receptor reconstitution research study synthetic peptide tool two-dimensional

项目摘要

The Protein Chemistry Laboratory Core will serve as the major protein preparation and analysis resource for the Program Project. The laboratory will be responsible for protein preparation, characterization and identification which will include hypothesis and project driven proteomics. The Protein Chemistry Laboratory is equipped with three automated peptide synthesizers, an automated Edman sequencer, various HPLC and FPLC systems encompassing both micro and macro scale. Most importantly the laboratory has two "proteomics-appropriate" mass spectrometry systems equipped with ionization modes that will enable sub-picomole analysis of proteins and peptides with bio-informatics support. All protein and peptide products will be subjected to stringent characterization by mass spectrometric/ proteomic analysis. Surface Plasmon resonance (Biacore) analysis is also available that is adaptable to coupling with the on-site mass spectrometers. The primary services provided will be the provision of protein-related tools and reagents such as purified murine complement system proteins, monoclonal and polyclonal antibodies, synthetic peptides, and peptide-like synthetic antagonists that interrupt various and specific complement system protein-protein interactions. Hypothesis driven proteomics will also be applied to delineate molecular and biochemical mechanisms underlying the various complement regulated disease models. All members of the program project will be served by the PCLC.

蛋白质化学实验室核心将作为主要的蛋白质制备和分析程序项目的资源。实验室将负责蛋白质制备，表征和识别将包括假设和项目驱动的蛋白质组学。这蛋白质化学实验室配备了三个自动肽合成器，一个自动化 Edman Sequencer，各种HPLC和FPLC系统，包括微型和宏观尺度。最重要的是，实验室具有两个“适合蛋白质组学”的质谱系统配备有电离模式，可以使蛋白质和肽的亚曲杆菌分析生物信息学支持。所有蛋白质和肽产品将受到严格的影响通过质谱/蛋白质组学分析来表征。表面等离子体共振（BIACORE）还可以使用分析，可与现场质谱仪结合使用。提供的主要服务将是提供与蛋白质相关的工具和试剂，例如纯化的鼠补体系统蛋白质，单克隆和多克隆抗体，合成肽和类似肽的合成拮抗剂，中断各种补体系统蛋白质 - 蛋白质相互作用。假设驱动的蛋白质组学也将用于划定各种补体调节疾病的基础的分子和生化机制型号。计划项目的所有成员将由PCLC服务。