Analysis of FGF Function in Limb Development

FGF在肢体发育中的功能分析

• 批准号: 6901004
• 负责人: Xin Sun
• 金额: $ 32.28万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6901004
• 关键词: biological signal transduction; developmental genetics; fibroblast growth factor; gene expression; gene interaction; gene mutation; genetically modified animals; growth factor receptors; histogenesis; laboratory mouse; limbs; protein structure function; transcription factor

DESCRIPTION (provided by applicant): The ultimate objective of this study is to understand the mechanism of vertebrate limb formation. The focus of this proposal is on limb development along its proximal-distal axis. Previous studies have established that members of the Fibroblast Growth Factor (FGF) family of secreted signaling molecules play a central role in this developmental context. However, their exact function has remained a disputed issue. Recent genetic data suggest that FGFs are important in establishing the proper size of progenitor populations prior to differentiation. In this study, genetic approaches will be employed to investigate the molecular and cellular basis of FGF function in the control of progenitor population size using mouse as a model organism. In this proposal, the validity of other previously proposed roles of FGFs in limb development would also be addressed. The data generated here will lead to a better understanding of the process of limb proximal-distal development. Since limb formation is a conserved process, our knowledge from the model organisms will provide insights into the basis for human limb defects, and the potential for regeneration of this external organ.

描述（由申请人提供）：这项研究的最终目标是了解脊椎动物肢体形成的机制。该提议的重点是沿其近端轴的肢体发育。先前的研究表明，在这种发育环境中，分泌信号分子的成纤维细胞生长因子（FGF）家族的成员起着核心作用。但是，它们的确切功能仍然是有争议的问题。最近的遗传数据表明，FGF在分化之前建立祖细胞种群的适当大小很重要。在这项研究中，将采用遗传方法来研究使用小鼠作为模型生物的小鼠控制FGF功能的分子和细胞基础。在此提案中，还将解决FGF在肢体发育中其他提议的作用的有效性。此处生成的数据将使人们更好地了解肢体近端发展过程。由于肢体形成是一个保守的过程，因此我们来自模型生物体的知识将为人类肢体缺陷的基础提供洞察力，以及这种外部器官的再生潜力。