Large Scale Sequencing and Analysis of Genomes

基因组大规模测序和分析

• 批准号: 6999359
• 负责人: ERIC S LANDER
• 金额: $ 4800.57万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-11-10 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6999359
• 关键词: cooperative study; gene expression; genome; molecular genetics; nucleic acid sequence

DESCRIPTION: (provided by applicant) The goal of this proposal is: To provide the scientific community with an unprecedented amount of assembled high-quality genome sequence, sufficient in quantity and quality to solve some of the central intellectual questions in genomics. In particular, the genome sequence will allow comparative studies to identify the vast majority of functional elements encoded in the human genome. It will also allow extensive characterization of the biological diversity in the animal and fungal kingdoms. We propose to do this, over a three-year period, by producing: Shotgun sequence: approximately 331 million reads yielding, approximately 205 Gb of high-quality raw bases, and approximately 32 Gb of high-quality draft genome assembly; Polished sequence: approximately 10 Gb; and Finished sequence: approximately 1.5 Gb. We also plan to aggressively improve the efficiency of the genome sequencing process. The cost of high quality draft sequence of a typical mammalian genome (about 2.7 Gb) will be approximately $25M in Year 1 and decrease to about $16M by Year 3.

描述：（由申请人提供）该提案的目标是： 为科学界提供前所未有的组装高质量基因组序列，其数量和质量足以解决基因组学中的一些核心知识问题。 特别是，基因组序列将使比较研究能够识别人类基因组中编码的绝大多数功能元件。 它还将允许对动物和真菌界的生物多样性进行广泛的表征。 我们建议在三年内通过以下方式实现这一目标： 鸟枪法序列：产生约 3.31 亿条读取，约 205 Gb 的高质量原始碱基，以及约 32 Gb 的高质量基因组草图组装； 抛光序列：约10 Gb；和 完成的序列：大约 1.5 GB。 我们还计划积极提高基因组测序过程的效率。典型哺乳动物基因组的高质量草图序列（约 2.7 Gb）的成本在第 1 年约为 2500 万美元，到第 3 年降至约 1600 万美元。