BIOSENSOR

生物传感器

• 批准号: 7313505
• 负责人: STEPHEN G. SLIGAR
• 金额: $ 7.5万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-10 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7313505
• 关键词: bioimaging /biomedical imaging; biological signal transduction; biomedical facility; biosensor device; cell migration; cooperative study; fluorescence resonance energy transfer; fluorescent dye /probe; guanine nucleotide binding protein; high throughput technology; imaging /visualization /scanning; intermolecular interaction; molecular polarity; phosphorylation; reporter genes; technology /technique development; time resolved data

The primary goals for the Biosensor Initiative are to continue to develop biosensors for key regulatory and effector nodes in cell migration and to use existing and proposed biosensors to acquire quantitative data that can be used to develop an experimentally verified theoretical model of cell migration by the Modeling Initiative and others in the modeling community. The Initiative builds on the sensor technologies, fluorescence assays, high throughput microscopy and collaborations from the first CMC funding period to move into Phase II, wherein we plan to visualize signaling events in living cells and to acquire and analyze large microscopic data sets. The modeling will be carried out as a close collaboration with the Modeling Initiative. A major focus of the Initiative will be Rho family regulators since they control key migration nodes. In addition the Initiative will focus on construction of biosensors and photoactivatable reagents, using both existing and new technologies, for key upstream and downstream members of these signaling pathways. In the end we produce a detailed experimental and mathematical analysis of a specific, critical signaling pathway in cell migration.

生物传感器计划的主要目标是继续开发用于细胞迁移中关键调节和效应节点的生物传感器，并使用现有和拟议的生物传感器来获取可用于 通过建模倡议和其他机构开发经过实验验证的细胞迁移理论模型 在模特界。该倡议建立在传感器技术、荧光分析、高 从第一个 CMC 资助期到进入第二阶段的通量显微镜和合作， 其中我们计划可视化活细胞中的信号事件并获取和分析大的微观 数据集。建模将与建模倡议组织密切合作进行。一个专业 该倡议的重点将是 Rho 家族监管机构，因为他们控制着关键的迁移节点。此外 该计划将侧重于利用现有的生物传感器和光活化试剂的构建 和新技术，用于这些信号通路的关键上游和下游成员。 最后，我们对特定的关键信号进行了详细的实验和数学分析 细胞迁移的途径。