Essential Human Embryonic Stem Cell Culture Methods

基本的人类胚胎干细胞培养方法

• 批准号: 7393740
• 负责人: MERI T FIRPO
• 金额: $ 11.93万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-12 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7393740
• 关键词: Appendix; Attention; Biology; Cells; Cellular biology; Class; Clinical; Communities; Depth; Disease; ES01; ES03; ES04; Education; Educational process of instructing; Ensure; Ethical Issues; Evaluation; Faculty; Fibroblasts; Freezing; Future; Genetic Drift; Growth; Hand; Human Resources; Individual; Institutes; Institution; Laboratories; Learning; MI01; Manuals; Methods; Minnesota; Modification; Mus; Participant; Pliability; Preparation; Principal Investigator; Procedures; Protocols documentation; Range; Research; Research Personnel; Resources; Schedule; Scientist; Site; Stem Cell Research; Stem cells; Suggestion; Techniques; Time; Training; Training Programs; Translational Research; UC01; UC06; Undifferentiated; Universities; adult stem cell; base; cell bank; clinical application; day; design; experience; follow-up; human embryonic stem cell; human embryonic stem cell line; interest; lecturer; lectures; member; programs; research facility; size; skills; stem cell technology; tool

DESCRIPTION (provided by applicant): This proposal outlines a plan to establish a human embryonic stem cell (hESC) training program at the Stem Cell Institute at the University of Minnesota. The program is designed to support the establishment of hESC studies in laboratories undertaking a broad range of research topics. The program draws upon of the depth of experience in stem cell research, and specifically hESC research at the UM Stem Cell Institute. Based on the extensive experience of the faculty of the program, and guest participants both from the UM Stem Cell Institute and outside speakers, the participants in the program will gain hands-on experience in the essential procedures required for hESC culture, and learn strategies for initiating hESC research in lectures presented by scientists actively engaged in stem cell and hESC research. The program consists of three sections, covered in a three-day training program to take place at the new site of the UM Stem Cell Institute. Five participants will be chosen for each of the four courses offered throughout the year. The main focus of the course is hands-on training on the preparation and use of feeder cultures and hESC. The hESC lines UC06 and WI09 will primarily be used, but lines UC01, WI01, MI01, ES01, ES03 and ES04 are also available for demonstration. The program also includes lecture sections covering differentiation, genetic modification and banking strategies. Finally, guest lecturers from the UM Stem Cell Institute, and guests from the research community outside of the University of Minnesota will describe research and clinical applications for stem cells. The small size of the classes will allow us to give individual attention to the participants' level of experience and research interests. Classes offered four times per year will provide participants flexibility in scheduling. Ongoing evaluation by the participants, and follow-up support by the program faculty will provide flexibility in refining subsequent classes. The faculty of this program have extensive experience in successfully training researchers in hESC technology for more than 5 years. This program establishes a valuable resource for the stem cell research community. This proposal outlines a plan to establish a training program in the use of human embryonic stem cells (hESC) for research. The program will train researchers to incorporate hESC studies into the research ongoing in their labs. The program consists of laboratory demonstrations, lectures on the use of hESC, and guest lectures on current and future applications of hESC research for biology and disease treatments.

描述（由申请人提供）：该提案概述了在明尼苏达大学干细胞研究所建立人类胚胎干细胞（hESC）培训计划的计划。该计划旨在支持在开展广泛研究课题的实验室中建立 hESC 研究。该计划借鉴了干细胞研究的深度经验，特别是 UM 干细胞研究所的 hESC 研究经验。基于该项目教师以及来自 UM 干细胞研究所的客座参与者和外部演讲者的丰富经验，该项目的参与者将获得 hESC 培养所需基本程序的实践经验，并学习 hESC 培养所需的策略。在积极从事干细胞和 hESC 研究的科学家的讲座中启动 hESC 研究。该计划由三个部分组成，涵盖在密歇根大学干细胞研究所新址进行的为期三天的培训计划。全年提供的四门课程每门都会选择五名参与者。该课程的主要重点是饲养层培养物和 hESC 的制备和使用的实践培训。主要使用 hESC 系 UC06 和 WI09，但也可使用 UC01、WI01、MI01、ES01、ES03 和 ES04 系进行演示。该计划还包括涵盖分化、基因改造和银行策略的讲座部分。最后，来自密歇根大学干细胞研究所的客座讲师和来自明尼苏达大学以外研究界的嘉宾将介绍干细胞的研究和临床应用。小班授课将使我们能够对参与者的经验水平和研究兴趣给予个别关注。每年提供四次课程将为参与者提供安排的灵活性。参与者的持续评估以及项目教师的后续支持将为完善后续课程提供灵活性。该项目的教师在成功培训 hESC 技术研究人员方面拥有超过 5 年的丰富经验。该计划为干细胞研究界建立了宝贵的资源。该提案概述了建立人类胚胎干细胞（hESC）研究培训计划的计划。该计划将培训研究人员将 hESC 研究纳入实验室正在进行的研究中。该计划包括实验室演示、有关 hESC 使用的讲座以及有关 hESC 研究当前和未来在生物学和疾病治疗方面的应用的客座讲座。