Liquid chromatography-mass spectroscopy (LC-MS) equipment for plant metabolism and host-pathogen interactions.

用于植物代谢和宿主-病原体相互作用的液相色谱-质谱 (LC-MS) 设备。

• 批准号: BB/F011652/1
• 负责人: Nicholas Smirnoff
• 金额: $ 31.78万
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF011652%2F1
• 关键词: Liquid; chromatography; mass; spectroscopy; LC

Liquid chromatography-mass spectroscopy (LC-MS) is a technique that allows separation and precise identification of a wide range of biologically-important molecules. The LC-MS will supplement gas chromatography-mass spectrometers and nuclear magnetic resonance spectrometers housed in the School of Biosciences at Exeter University, to provide an analytical facility for biomolecules. The LC-MS will be used to support a wide range of research projects in the area of plant and microbial sciences. The projects to be supported and enabled include the following. 1. Investigations of the synthesis and function of vitamin C in plants and of the genetic control of metabolism. 2. The response of plants to fungal and bacterial pathogens: what kinds of chemical signals and defensive chemicals are involved in disease resistance. 3. Toxin production by pathogenic bacteria. 4. Algal metabolism: algae (phytoplankton) are important for photosynthesis and productivity in the oceans and are key in absorbing carbon dioxide from the atmosphere. Studies of algal metabolism in conjunction with new genome sequence information will provide new information on their response to climate change.

液相色谱-质谱 (LC-MS) 是一种能够分离和精确鉴定多种重要生物学分子的技术。 LC-MS 将补充埃克塞特大学生物科学学院的气相色谱-质谱仪和核磁共振光谱仪，为生物分子提供分析设施。 LC-MS 将用于支持植物和微生物科学领域的广泛研究项目。将支持和启用的项目包括以下内容。 1.植物中维生素C的合成和功能以及代谢遗传控制的研究。 2.植物对真菌和细菌病原体的反应：哪些化学信号和防御化学物质参与抗病性。 3.病原菌产生毒素。 4.藻类代谢：藻类（浮游植物）对于海洋的光合作用和生产力非常重要，并且是吸收大气中二氧化碳的关键。结合新的基因组序列信息对藻类代谢的研究将为它们对气候变化的响应提供新的信息。

项目成果

Jasmonates induce Arabidopsis bioactivities selectively inhibiting the growth of breast cancer cells through CDC6 and mTOR.

茉莉酸诱导拟南芥生物活性，通过 CDC6 和 mTOR 选择性抑制乳腺癌细胞的生长。

• DOI: http://dx.10.1111/nph.17031
• 发表时间: 2021
• 期刊: The New phytologist
• 作者: Bömer M

Responses of a Newly Evolved Auxotroph of Chlamydomonas to B12 Deprivation.

新进化的衣藻营养缺陷体对 B12 缺乏的反应。

• DOI: http://dx.10.1104/pp.19.01375
• 发表时间: 2020
• 期刊: Plant physiology
• 影响因子: 7.4
• 作者: Bunbury F

Quantitative proteomics of a B12 -dependent alga grown in coculture with bacteria reveals metabolic tradeoffs required for mutualism.

与细菌共培养的 B12 依赖性藻类的定量蛋白质组学揭示了互利共生所需的代谢权衡。

• DOI: http://dx.10.1111/nph.14832
• 发表时间: 2018
• 期刊: The New phytologist
• 作者: Helliwell KE

Differential sensitivity of honey bees and bumble bees to a dietary insecticide (imidacloprid).

蜜蜂和熊蜂对膳食杀虫剂（吡虫啉）的不同敏感性。

• DOI: 10.1016/j.zool.2012.05.003
• 发表时间: 2012-12-01
• 期刊: Zoology
• 影响因子: 2
• 作者: J. Cresswell;Christopher J Page;Mehmet Uygun;Marie Holmbergh;Yueru Li;Jonathan G Wheeler;I. Laycock;C. Pook;N. H. de Ibarra;N. Smirnoff;C. Tyler
• 通讯作者: C. Tyler

Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis.

时间序列转录组学揭示 AGAMOUS-LIKE22 影响干旱胁迫拟南芥的初级代谢和发育过程。

• DOI: http://dx.10.1105/tpc.15.00910
• 发表时间: 2016
• 期刊: The Plant cell
• 作者: Bechtold U