Signal Transduction in Nitrogenase Fe Protein

固氮酶 Fe 蛋白的信号转导

基本信息

批准号：
7157679
负责人：
Michael Alan Vance
金额：
$ 4.4万
依托单位：
MONTANA STATE UNIVERSITY - BOZEMAN
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2009-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7157679
关键词：
X ray crystallography adenosine triphosphate aminoacid bioenergetics biological signal transduction calorimetry conformation enzyme complex enzyme mechanism hemoprotein hydrolysis low angle X ray diffraction analysis magnesium mutant nitrogenase nucleotides postdoctoral investigator quantum chemistry

项目摘要

DESCRIPTION (provided by applicant): Signal transduction, an important mechanism in a variety of cellular processes (e.g. protein biosynthesis, hormone stimulated metabolic activities, muscle contraction, etc.), is carried out in part through protein conformational changes induced by nucleoside triphosphate binding and hydrolysis. The Fe protein in nitrogenase is one member of this class of proteins. Although structures exist for nitrogenase component proteins, the factors affecting conformational change and MgATP hydrolysis are not well understood. The major objectives of this proposal are to use biochemical, biophysical, and computational techniques (x- ray crystallography, small angle x-ray scattering, calorimetry, and QM/MM calculations) to define conformational changes in the Fe protein during nucleotide binding, hydrolysis, and signal transduction as well as effects of amino acid environment on modulating [4Fe-4S] cluster properties.

描述（由申请人提供）：信号转导是多种细胞过程（例如蛋白质生物合成、激素刺激的代谢活动、肌肉收缩等）中的重要机制，部分是通过三磷酸核苷结合诱导的蛋白质构象变化来实现的和水解。固氮酶中的铁蛋白是此类蛋白质的成员之一。尽管固氮酶成分蛋白存在结构，但影响构象变化和 MgATP 水解的因素尚不清楚。该提案的主要目标是使用生物化学、生物物理和计算技术（X 射线晶体学、小角度 X 射线散射、量热法和 QM/MM 计算）来定义 Fe 蛋白在核苷酸结合、水解过程中的构象变化。、信号转导以及氨基酸环境对调节 [4Fe-4S] 簇特性的影响。