MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

基本信息

批准号：
7467917
负责人：
Arion Kennedy
金额：
$ 2.5万
依托单位：
UNIVERSITY OF NORTH CAROLINA GREENSBORO
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-07-15 至 2009-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7467917
关键词：
1,2-diacylglycerol 20 year old Adipocytes Adipose tissue Adolescent Adult Adverse effects Americas Animals Attenuated Body Weight Body Weight decreased Calcium Cardiovascular Diseases Cell model Chelating Agents Child Chronic Disease Conjugated Linoleic Acids Cytokine Suppression Dairy Products Data Deposition Development Dietary Fats Dietary Supplementation Diglycerides Disease Eicosanoids Energy Intake Energy Metabolism Enzymes Epidemic Extracellular Signal Regulated Kinases Fatty Acids Fatty acid glycerol esters Fellowship Program Fortified Food Gene Expression Gene Proteins Gene Targeting Glucose Goals Health Health Care Costs Human Immunoblotting Incidence Inflammation Inositol Insulin Resistance Interleukin-6 Interleukin-8 Isoenzymes Isomerism Knowledge Lead Linoleic Acids Lipase Lipids Meat Mediating Medical Messenger RNA Minority Mitogen-Activated Protein Kinases Modeling NF-kappaB-inducing kinase Non-Insulin-Dependent Diabetes Mellitus Nuclear Nutritional Obesity Outcome Overweight Peroxisome Proliferator-Activated Receptors Phospho-Specific Antibodies Phospholipase A2 Phospholipase C Phosphorylation Phosphotransferases Prevention Production Property Prostaglandin-Endoperoxide Synthase Prostaglandins Protein Kinase C Protein Kinase C Inhibitor Publishing Regulation Relative (related person)Reporting Research Research Personnel Research Project Grants Risk Factors Role Ruminants Safety Signal Transduction Specificity Stream Stroke Supplementation Testing Triglycerides Tumor Necrosis Factor-alpha Tumor Necrosis Factors Work aged base beef citrate carrier cyclooxygenase 1 cyclooxygenase 2 cytokine dietary supplements enzyme activity extracellular glucose uptake human TNF protein human study inhibitor/antagonist innovation insight lipid metabolism novel obesity treatment pre-doctoral prevent programs protein expression response therapeutic target uptake user-friendly

项目摘要

DESCRIPTION (provided by applicant): The long-term goal of this research project is to identify novel dietary strategies for controlling human obesity, the most prevalent nutritional-related disease in America. The objective of this application is to identify upstream signals produced by an isomer of conjugated linoleic acid (CLA), fatty acids found in beef and dairy products and in dietary supplements for weight loss, that activate cell signals that lead to adipocyte delipidation. To complete this objective, the following specific aims will be carried out in cultures of human adipocytes: Aim #1) Determine the role of protein kinase C (PKC) in mediating CLA's activation of nuclear factor KB (NFKB) and extracellular signal-regulated kinase kinase (ERK)1/2 and delipidation; Aim #2) Identify the mechanism by which CLA increases [Ca2+]i, and its impact on NFKB and ERK1/2 signaling and delipidation; and Aim #3) Determine the extent to which CLA increases the activities of lipid metabolizing enzymes, generating signals that activate NFKB and ERK1/2 and promote delipidation. Data attained from this proposal are expected to provide critical insight for the development of therapeutic targets for the prevention and treatment of obesity and type II diabetes, and reduce health care costs related to obesity.

描述（由申请人提供）：该研究项目的长期目标是确定控制人类肥胖（美国最普遍的营养相关疾病）的新饮食策略。该应用的目的是识别共轭亚油酸 (CLA) 异构体产生的上游信号，这种脂肪酸存在于牛肉和乳制品以及减肥膳食补充剂中，这些信号激活细胞信号，导致脂肪细胞脱脂。为了实现这一目标，将在人类脂肪细胞培养物中实现以下具体目标：目标#1) 确定蛋白激酶 C (PKC) 在介导 CLA 激活核因子 KB (NFKB) 和细胞外信号调节激酶中的作用激酶 (ERK)1/2 和脱脂；目标 #2) 确定 CLA 增加 [Ca2+]i 的机制及其对 NFKB 和 ERK1/2 信号传导和脱脂的影响；目标 #3) 确定 CLA 增加脂质代谢酶活性的程度，产生激活 NFKB 和 ERK1/2 并促进脱脂的信号。从该提案中获得的数据预计将为预防和治疗肥胖和 II 型糖尿病的治疗目标的开发提供重要的见解，并降低与肥胖相关的医疗保健成本。