MARC Predoctoral Fellowship Program

MARC 博士前奖学金计划

• 批准号: 7354064
• 负责人: Jenel Nixon
• 金额: $ 3.07万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7354064
• 关键词: Address; Binding; Binding Proteins; Biological Assay; Biological Models; CCL7 gene; Cell Nucleus; Chimera organism; Chromatin; Chromosome Mapping; DNA; DNA Packaging; Defect; Eukaryota; Eukaryotic Cell; Fellowship Program; Fluorescence Microscopy; Gene Deletion; Gene Expression; Gene Expression Regulation; Genes; Genome; Goals; Grant; Heterochromatin; Human; Lamins; Lead; Learning; Libraries; Localized; Mediating; Membrane Proteins; Monitor; Muscular Dystrophies; Mutation; Nuclear; Nuclear Envelope; Nuclear Lamin; Nuclear Pore; Pathway interactions; Plasmids; Positioning Attribute; Progeria; Proteins; Purpose; Role; Saccharomyces; Screening procedure; Structure; Syndrome; Transcriptional Regulation; Ubiquitin; Yeasts; base; deletion library; gene function; human disease; mutant; pre-doctoral; research study; transcription factor; yeast two hybrid system

DESCRIPTION (provided by applicant): The packaging of DNA in chromatin greatly influences gene expression. Recent findings indicate that chromatin positioning within the nucleus influences gene activity. In particular, the nuclear periphery appears to comprise a domain critical for transcriptional control. Many highly expressed genes localize to nuclear pores. Silenced chromatin also occupies a specific nuclear address. In yeast and in higher eukaryotes, silenced chromatin resides in sub-compartments at the nuclear periphery. In humans, some mutation of lamins, genes that contribute to the nuclear compartmentalization of chromatin, lead to laminopathies, including muscular dystrophy and Hutchinson-Gilford Progeria Syndrome. The goal of this proposal is to understand how chromatin organization is determined using yeast Saccharomyces as a model system. The Gartenberg lab recently showed that yeast heterochromatin is bound to the nuclear periphery by a nuclear membrane-associated protein Esc1.I aim to identify Esc1 interacting partners by adapting the split ubiquitin yeast two hybrid screen and by using and a whole genome cytological screen. I will then use silencing and DNA localization assays to learn how Esc1 contributes to the nuclear position and regulation of genes.

描述（由申请人提供）：染色质中DNA的包装极大地影响了基因表达。最近的发现表明，核中染色质的位置会影响基因活性。 特别是，核外围似乎包括一个对转录控制至关重要的领域。 许多高度表达的基因本地化为核孔。 沉默的染色质也占据了特定的核地址。 在酵母和较高的真核生物中，沉默的染色质位于核外围的子室中。 在人类中，一些层粘连蛋白的突变，导致染色质的核分室化的基因会导致椎板病，包括肌肉营养不良和Hutchinson-Gilford Progeria综合征。 该提案的目的是了解如何使用酵母糖疗法作为模型系统确定染色质组织。 Gartenberg实验室最近表明，酵母异染色质通过核膜相关蛋白ESC1与核周围结合。I旨在通过适应分裂的泛素酵母菌酵母两个混合筛选并使用和使用和整个基因组细胞学学筛查来鉴定ESC1相互作用伴侣。 然后，我将使用沉默和DNA定位分析来了解ESC1如何促进基因的核位置和调节。