Pathogen Diagnostics with 3D Protein Nanotube-Chip Arrays

使用 3D 蛋白质纳米管芯片阵列进行病原体诊断

• 批准号: 7391707
• 负责人: Hiroshi Matsui
• 金额: $ 19.62万
• 依托单位: HUNTER COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7391707
• 关键词: 3-Dimensional; Address; Antibodies; Antigens; Architecture; Binding; Biohazardous Substance; Biological; Biological Assay; Bioterrorism; Characteristics; Color; Condition; Country; DNA; Data; Deposition; Detection; Devices; Diagnosis; Diagnostic; Diagnostic Procedure; Dimensions; Discrimination; Disease; Electrodes; Event; Fingers; Food; Image; Ions; Liquid substance; Measures; Metals; Methods; Microscopic; Molecular; Nanostructures; Nanotubes; Organism; Output; Pattern; Performance; Placement; Play; Positioning Attribute; Process; Protein Array; Protein Microchips; Proteins; Reproducibility; Request for Proposals; Role; Sampling; Sensitivity and Specificity; Signal Transduction; Solutions; Surface; System; Time; War; antibody conjugate; base; cost; density; design; improved; innovation; light weight; nano; nanoscale; nanostructured; pathogen; real world application; self assembly; size; two-dimensional; uptake

DESCRIPTION (provided by applicant): Bioterrorism is becoming a major threat for many countries as a new style of war. To protect the public from such a biological attack, we need to establish improved diagnostic methods and sampling strategies in order to identify the pathogens more rapidly and precisely. We hypothesize that a smart 3-D arrayed protein nanotube chip, can diagnose a single binding event of a target pathogen in a flowed liquid by using a combination of the biological recognition functions of antibodies conjugated onto the ends of nanotubes and size discrimination such that the bound target must bridge a pre-defined nanotube-Au substrate gap to complete the circuit. Subsequently, specific confirmation of target identity will be conducted through microbiological or molecular identification steps. The target concentration will be estimated in real time by conductivity change as the target pathogen bridges the gap between the nanotube and the second electrical terminal comprising an Au substrate. This identification based on fulfilling two characteristics (presence of antigen and physical size) is an innovative method because A) A positive signal will require two parameters to be satisfied, i.e. size and antigenicity, and therefore it will reduce 'false positive signals' from cell fragments, food matrices, and environmental materials in which pathogens reside; B) it provides electrical signals in real-time; C) it is non-destructive, permitting subsequent analyses; D) it does not amplify potential biohazard; E) it can be stacked allowing flowed samples and multiple targets to be addressed; and F) it is potentially widely deployable.

描述（由申请人提供）：生物恐怖主义已成为许多国家的主要威胁 作为一种新的战争风格。为了保护公众免受这种生物攻击的侵害，我们需要建立 改进的诊断方法和抽样策略，以更快，更精确地识别病原体。 We hypothesize that a smart 3-D arrayed protein nanotube chip, can diagnose a single binding event of a target pathogen in a flowed liquid by using a combination of the biological recognition functions of antibodies conjugated onto the ends of nanotubes and size discrimination such that the bound target must bridge a pre-defined nanotube-Au substrate gap to complete the circuit.随后，将通过微生物或分子识别步骤进行特定的目标身份确认。当目标病原体桥接纳米管与包括AU底物的第二个电端子之间的间隙时，目标浓度将通过电导率变化实时估计。这种基于实现两个特征（抗原和物理大小的存在）的识别是一种创新方法，因为a）正信号将需要两个参数满足，即大小和抗原性，因此它将从细胞片段，食物矩阵，食物矩阵和环境材料中降低“假阳性信号”，而病原体居住； b）它实时提供电信号； c）它是无损的，允许随后的分析； d）它不会扩大潜在的生物危害； e）它可以堆叠以允许流动样品和多个目标 要解决； f）它可能在可部署。