Spinning disc confocal microscope for live cell imaging

用于活细胞成像的转盘共聚焦显微镜

• 批准号: 7213222
• 负责人: LYNNE M COLUCCIO
• 金额: $ 45.71万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-22 至 2009-03-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7213222
• 关键词: Advisory Committees; Apoptosis; Area; Biomedical Research; Boston; Cells; Cellular biology; Core Facility; Diabetes Mellitus; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Heart Diseases; Image; Injury; Institutes; Laser Scanning Confocal Microscopy; Lead; Life; Maintenance; Malignant Neoplasms; Membrane; Microscope; Motion; Muscle Development; Myocardial; Neuromuscular Diseases; Phosphatidylinositols; Regulation; Research; Research Institute; Research Personnel; Research Project Grants; Role; Signal Transduction; Signaling Protein; Smooth Muscle; Standards of Weights and Measures; Structure-Activity Relationship; Support Groups; Support of Research; System; Time; Training; cell motility; cellular imaging; cyclic GMP-binding protein; instrument; instrumentation; novel; protein structure function; response

DESCRIPTION (provided by applicant): Ten NIH-supported group leaders and their research staff at Boston Biomedical Research Institute (BBRI) request funds for the purchase of a Perkin Elmer UltraVIEW ERS FRET O spinning disc imaging system for confocal imaging of live cells. These investigators are involved in a variety of NIH-supported research projects requiring live cell imaging capability including Ca2+ signaling, cytoskeletal regulation, membrane dynamics, cellular responses to myocardial ischemic injury, phosphoinositide signaling, the role of novel cGMP binding proteins, and the structure-function relationships of contractile and signaling proteins in smooth muscle. The requested microscope with dedicated camera system allows for rapid confocal image acquisition, suitable for recording fast sequences of motions in live cells, as well as long time-courses, features not duplicated by standard fluorescence or traditional scanning confocal microscopy. The application is supported by considerable preliminary studies showing the suitability of the requested instrument for the ongoing studies. The microscope will be incorporated into the Cell Biology Core Facility. Maintenance and training of users will be provided by the PI and institute-supported technical staff. An advisory committee will be responsible for administrative and scientific oversight. The studies to be supported by the requested instrumentation will lead to ground-breaking discoveries in research areas including cell migration, apoptosis, cell signaling, and muscle development; and to understanding cancer, heart disease, pre-term labor, diabetes, and neuromuscular diseases among others.

描述（由申请人提供）：波士顿生物医学研究所 (BBRI) 的 10 名 NIH 支持的小组领导及其研究人员请求资金购买 Perkin Elmer UltraVIEW ERS ​​FRET O 旋转盘成像系统，用于活细胞共聚焦成像。这些研究人员参与了 NIH 支持的各种需要活细胞成像能力的研究项目，包括 Ca2+ 信号传导、细胞骨架调节、膜动力学、细胞对心肌缺血损伤的反应、磷酸肌醇信号传导、新型 cGMP 结合蛋白的作用以及结构 -平滑肌收缩蛋白和信号蛋白的功能关系。所要求的带有专用摄像系统的显微镜可实现快速共焦图像采集，适合记录活细胞中的快速运动序列以及长时间进程，这些功能是标准荧光或传统扫描共焦显微镜无法复制的。该申请得到了大量初步研究的支持，表明所需仪器适合正在进行的研究。该显微镜将被纳入细胞生物学核心设施。用户的维护和培训将由PI和研究所支持的技术人员提供。咨询委员会将负责行政和科学监督。由所需仪器支持的研究将在细胞迁移、细胞凋亡、细胞信号传导和肌肉发育等研究领域带来突破性的发现；并了解癌症、心脏病、早产、糖尿病和神经肌肉疾病等。