Prevention of Cartilage Degeneration Associated with Meniscal Injury

预防与半月板损伤相关的软骨退变

基本信息

批准号：
7486879
负责人：
RANDY N ROSIER
金额：
$ 43.46万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7486879
关键词：
Acute Address Aging Animal Model Arthritis Arthroscopy Basic Science Bone and Cartilage Funding Cartilage Cartilage injury Cells Chondrocytes Clinical Clinical Research Coix Condition Data Debridement Degenerative polyarthritis Development Diagnostic Disease Disease Progression Evaluation Event Fracture Healing Future Gene Expression Genes Genetic Genetic Models Human Image Immunohistochemistry In Situ Hybridization Inflammatory Injury Interleukin-1 Joints Knee Knock-out Lasers Lead Lesion Link Magnetic Resonance Imaging Matrix Metalloproteinases Measures Medial Membrane Meniscus structure of joint Mesenchymal Methodology Methods Modeling Molecular Mus Operative Surgical Procedures Orthopedics Osteocalcin Outcome Measure PTHLH gene Pathogenesis Pathway interactions Patients Phenotype Polymerase Chain Reaction Prevention Process Proteoglycan Regulation Research Personnel Residual state Role Seminal Signal Pathway Signal Transduction Skeletal system TNF gene Therapeutic Intervention Time Tissues Transcriptional Activation Transforming Growth Factor beta Transgenes Transgenic Mice Translating Trauma Ubiquitin Up-Regulation articular cartilage base bone bone cell bone loss bone morphogenetic protein 6 cytokine disease phenotype functional outcomes gene therapy injury and repair interest novel parathyroid hormone-related protein prognostic programs research study skeletal injury therapeutic target ubiquitin ligase

项目摘要

In the context of orthopaedic trauma, this project proposes to evaluate the relationship between meniscal injury and the development of osteoarthritis (OA). A significant clinical association has been documented between traumatic meniscal injury and OA, but the mechanism(s) behind how damage to the meniscus either directly or indirectly induces pathogenesis are not known. Recently, we have determined that articular chondrocyte loss of TGF-beta signaling induced by over-expression of the ubiquitin ligase Smurf2 leads to an OA-like phenotype in the mouse. Furthermore, we have identified up-regulation of Smurf2 in human articular cartilage shortly following meniscal trauma. Based on these findings, we hypothesize that Smurf2 up-regulation is the seminal event in the arthritic process the follows meniscal injury. Furthermore, based on our findings that increased BMP signaling occurs in conjunction with inappropriate maturation of articular chondrocytes during OA, we also hypothesize that reduction of BMP signaling via genetic or gene therapy approaches will decelerate disease progression in murine OA induced by meniscal injury. To address these central hypotheses, we propose to address the following 3 Specific Aims: In Aim 1, we will comprehensively characterize the tissue and molecular events leading to cartilage degeneration in a model of murine OA induced by meniscal injury. In Aim 2, we will use genetic and gene therapy approaches to evaluate a candidate therapeutic intervention in this murine OA model that are based on reduction of BMP signaling. For these basic science aims, we will employ MRI and microCT imaging methods, histomorphometry and molecular analyses to evaluate disease phenotype. Then, in Aim 3, a human clinical study will be executed which will quantify articular cartilage structural changes following acute meniscal injury using a quantitative MRI approach. Molecular changes will also be assessed in discard cartilage and meniscus tissue to further evaluate the involvement of Smurf2 in the pathogenesis of OA disease following injury.

在骨科创伤的背景下，该项目提议评估半月板之间的关系损伤和骨关节炎（OA）的发展。已经记录了重要的临床协会在半月板伤害和OA之间直接或间接诱导发病机理尚不清楚。最近，我们确定了关节泛素连接酶Smurf2诱导的TGF-β信号传导的软骨细胞损失导致小鼠中的OA样表型。此外，我们已经确定了人类蓝精灵的上调在半月板创伤后不久，关节软骨。基于这些发现，我们假设Smurf2 上调是关节炎过程中的开创性事件。此外，基于我们的发现增加了BMP信号传导与关节的不当成熟结合 OA期间的软骨细胞，我们还假设通过遗传或基因治疗减少BMP信号传导方法将在半月板损伤引起的鼠OA中减速疾病进展。解决这些中心假设，我们建议解决以下3个具体目标：在AIM 1中，我们将全面表征在鼠OA模型中导致软骨变性的组织和分子事件由半月板受伤引起。在AIM 2中，我们将使用遗传和基因治疗方法评估基于BMP信号的降低，该鼠OA模型中的候选治疗干预措施。对于这些基础科学的目的，我们将采用MRI和MICROCT成像方法，组织形态法和分子分析以评估疾病表型。然后，在AIM 3中，将执行人类的临床研究它将使用定量进行急性半月板损伤后量化关节软骨结构变化 MRI方法。分子变化也将在丢弃软骨和半月板组织中进行评估以进一步评估Smurf2受伤后OA疾病的发病机理的参与。