A state of the art multiparametric flow cytometry analysis system for multidisciplinary stem cell research

用于多学科干细胞研究的最先进的多参数流式细胞术分析系统

基本信息

批准号：
BB/E012841/1
负责人：
Majlinda Lako
金额：
$ 32.05万
依托单位：
Newcastle University
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2007
资助国家：
英国
起止时间：
2007 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FE012841%2F1
关键词：
state art multiparametric flow cytometry

项目摘要

Stem cells are capable of essentially extensive growth whilst retaining the ability to differentiate into most of the cell types found in the adult but unless we can find ways to grow them in large numbers while directing them to differentiate into therapeutically useful cells much of this potential will not be realised. A major obstacle impacting the derivation of differentiated cell types from stem cells is that current in vitro differentiation strategies often only produced the desired cells in small numbers. This means they must be separated from the other cell types present and one of the best techniques we have for doing this is flow cytometry. This technology relies upon the binding of fluorescent labelled antibodies to the surfaces of our desired cells such that when they pass through the flow cytometer they can be separated from the others and retain their viability. If appropriate antibodies are available, up to 12 different colours can be analysed at any one time using the instrumentation detailed in this proposal and this offers us an unparalleled advantage in cell identification and separation. There are a number of active research groups at the Institute of Human Genetics investigating a range of differentiation protocols designed to produce useful cells from a range of stem cells and flow cytometry instrumentation is not only indispensable to this work but is also subject to very heavy demand. In view of this, a successful outcome of this proposal would greatly enhance our ability to develop useful techniques for stem cell differentiation that will ultimately benefit medical science in general.

干细胞基本上能够广泛生长，同时保留分化成成人中发现的大多数细胞类型的能力，但除非我们能找到大量培养它们的方法，同时指导它们分化成治疗上有用的细胞，否则这种潜力的大部分将无法实现。不被实现。影响从干细胞衍生出分化细胞类型的一个主要障碍是，当前的体外分化策略通常只能产生少量所需的细胞。这意味着它们必须与存在的其他细胞类型分开，而我们拥有的最好的技术之一就是流式细胞术。该技术依赖于荧光标记抗体与我们所需细胞表面的结合，这样当它们通过流式细胞仪时，它们可以与其他细胞分离并保留其活力。如果有合适的抗体，使用本提案中详细介绍的仪器可以同时分析多达 12 种不同的颜色，这为我们在细胞识别和分离方面提供了无与伦比的优势。人类遗传学研究所有许多活跃的研究小组，研究一系列旨在从一系列干细胞中产生有用细胞的分化方案，流式细胞仪仪器不仅是这项工作不可或缺的，而且需求量非常大。有鉴于此，该提案的成功结果将极大地增强我们开发干细胞分化有用技术的能力，这最终将有利于整个医学科学。