Cell, Animal and Imaging Core

细胞、动物和成像核心

• 批准号: 7728884
• 负责人: THERESA A GUISE
• 金额: $ 12.23万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7728884
• 关键词: American Type Culture Collection; Animal Experiments; Animal Model; Animals; Behavior; Biological Assay; Bladder; Bone neoplasms; Cell Line; Cell physiology; Cells; Cellular Structures; Common Neoplasm; Cultured Cells; Data; Data Quality; Databases; Detection; Documentation; Endothelial Cells; Equipment; Fingerprint; Firefly Luciferases; Freezing; Genetic screening method; Goals; Guidelines; Histology; Housing; Human Cell Line; Human Resources; Image; Image Analysis; Injection of therapeutic agent; Instruction; Laboratories; Lead; Life; Luciferases; Malignant neoplasm of prostate; Manuscripts; Metastatic Neoplasm to the Bone; Microscopy; Modality; Morphology; Mus; Mycoplasma; Nature; Neoplasm Metastasis; Orchiectomy; Organ Culture Techniques; Osteoblasts; Osteoclasts; Osteolytic; Phenotype; Procedures; Process; Prostate; Reagent; Reporter; Research; Research Personnel; Resources; Risk; Sampling; Source; Standardization; Standards of Weights and Measures; System; Techniques; Testing; Tissues; Training Programs; Tumor-Derived; Xenograft Model; Xenograft procedure; angiogenesis; base; bone; experience; immortalized cell; improved; in vivo; macromolecule; member; mouse model; neoplastic cell; programs; promoter; repository; single cell analysis; subcutaneous; tissue culture; tumor

The Program makes extensive use of human cell lines and animal models of prostate cancer. The overall goal of this Core is to provide materials, instructional support, technical aid,/n vivo imaging of mouse models of prostate cancer metastases, and ex-vivo assays of tumor-bone interactions to Project investigators. This will encompass the storage and distribution of well-characterized prostate cell lines and xenografts; the storage and distribution of reporter and inducible cell lines; and instruction, guidelines and assistance with xenograft studies, Xenogen and Faxitron imaging and assays for osteoblast, osteoclast and angiogenesis. Such standardization of cell culture reagents and animal techniques will facilitate research by improving the quality of the data, reducing redundancy and allowing comparison of results between the all Projects. This Core will have 5 Aims: 1) Provide wellcharacterized prostate cancer and normal prostate cell lines to investigators and house a repository of all common tumor and immortalized cell lines. In addition, the Core will carry out mycoplasma testing and genetic fingerprinting (SNP analysis and/or VNTRs) of all cell lines received by Program investigators from outside sources. Thus, any cell line received by an investigator will first be processed by the Core in this way and a sample frozen in the repository. The Core will also serve as a repository of new cell lines derived by Program investigators such as those with inducible promoters (S. Parsons, Weber) or with imaging capability such as those containing transfected luciferase constructs (Theodorescu). 2) Provide live instruction and guidelines for investigators using xenograft models such as those derived after subcutaneous, intracardiac, intrafemoral and intraprostatic injection of tumor cells and with procedures such as orchiectomy. Instructional videos will be made of these procedures and saved on a central server database (PROSTA, Core A) accessible by all Program members via standard networked PCs. 3) Support for in vivo fluorescent imaging of tumors and Faxitron radiographic imaging and quantitation of bone metastases will be provided by the Core. Technical help will involve providing instruction as well as taking animals to the UVA SAMMIC core for MS (Xenogen Corp.) imaging of tumors expressing luciferase. 4) Assessment of tumor-bone interactions will be performed using wellcharacterized assays of osteoblast, osteoclast and endothelial cell function. 5) Microscopy Imaging: a) Resources for the routine analysis and documentation of cell structure and morphology and the detection of macromolecules in fixed, live cells and tissues; b) resources for single cell analysis; and c) training for Program personnel and overall oversight of Core equipment.

该计划广泛使用人类细胞系和前列腺癌的动物模型。总体目标 该核心是提供材料，教学支持，技术援助，/N Vivo鼠标模型的前列腺模型 癌症转移酶和对项目研究人员肿瘤骨相互作用的前体病毒测定法。这将包括 特征良好的前列腺细胞系和异种移植物的存储和分布；存储和分布的 记者和诱导细胞系；以及有关异种移植研究的指导，指南和协助，异种和 Faxitron成像和成骨细胞，破骨细胞和血管生成的测定。细胞培养的标准化 试剂和动物技术将通过提高数据质量来促进研究，从而降低冗余 并可以比较所有项目之间的结果。该核心将有5个目标：1）提供良好的表演 前列腺癌和正常前列腺细胞系向研究者，并容纳所有常见的存储库 肿瘤和永生的细胞系。此外，核心将进行支原体测试和遗传 计划调查人员从外部收到的所有细胞系的指纹分析（SNP分析和/或VNTR） 来源。因此，研究人员收到的任何单元线将首先由核心以这种方式处理 样品在存储库中冷冻。核心还将作为程序得出的新单元线的存储库 诸如具有诱导启动子（S. Parsons，Weber）或具有成像能力的研究者（例如 包含转染的荧光素酶构建体（Theodorescu）。 2）提供实时指导和指南 使用异种移植模型的研究者，例如皮下，心脏内，财富和 肉体内注射肿瘤细胞以及诸如鸡蛋切除术等程序。将制作教学视频 在这些过程中，并保存在中央服务器数据库（prosta，core a）都可以通过所有程序访问 通过标准网络PC的成员。 3）支持肿瘤的体内荧光成像 核心将提供骨转移的影像学成像和定量。技术帮助将涉及 提供指导以及将动物带到MS的UVA Sammic核心（异源Corp.）成像 表达荧光素酶的肿瘤。 4）将使用良好的表演进行评估肿瘤骨相互作用 成骨细胞，破骨细胞和内皮细胞功能的测定。 5）显微镜成像：a）资源 用于常规分析和记录细胞结构和形态以及大分子的检测 在固定的活细胞和组织中； b）单细胞分析的资源； c）培训计划人员和 核心设备的总体监督。