Long Term Storage and Precision Dispensing of Precious Liquids

贵重液体的长期储存和精确分配

• 批准号: 7106733
• 负责人: GORDON Ian ROBERTSON
• 金额: $ 10万
• 依托单位: ARCHIVEX, LLC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-09-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7106733
• 关键词: animal genetic material tag; biomedical automation; biomedical registry /referral center; chemical standardization; genetic registry /resource /referral center; human genetic material tag; preservation; technology /technique development

DESCRIPTION (provided by applicant): Integrated disease phenotype and cellular genotype research is advanced by testing hypotheses using stored aqueous samples of DNA, cDNA or other precious liquids from subjects with known characteristics. Many life science laboratories store thousands of such aqueous samples for years or decades, and these researchers rely absolutely on accurate inventory knowledge and known provenance of the samples. The methods used today for storing these precious liquid samples are ad-hoc and prone to contamination and mis-identification. This reduces the productivity and satisfaction of researchers in a variety of ways. The goal of this project is to develop a system for long term storage of precious liquids in standardized containers with machine-readable identification for positive and unambiguous identification of their contents. An automated precision dispenser which does not require pipette intrusion will ensure sample integrity while dispensing microliter aliquots of precious liquid. An interface between the automated dispensing equipment and the laboratory database will maintain accurate inventory control and allow improved experiment planning. This system will greatly increase the efficiency and reduce the error rate of genomic research by ensuring that the precious liquid samples are contamination-free, maintain their concentration levels, and are correctly and positively identified. When completed, the system will be commercialized by marketing it to life-science laboratories, forensic laboratories and hospitals. Technical innovations in this program are the patented design of the standardized storage container; a novel and proprietary means for dispensing 1 ul drops without contamination, and the container identification method and database interface protocols. During Phase 1 of the project, prototypes of the disposable container with machine-readable ID will be designed and built (Specific Aim 1), the proprietary new drop dispensing technology will be developed (Specific Aim 2), and software interface protocols for communicating between the system and the laboratory database will be planned (Specific Aim 3). As each prototype is built, it will be alpha tested in an operating life-science laboratory. During Phase 2, a fully automated system with direct interface to the user laboratory database will be constructed. Public health will directly benefit from the results of the proposed work by reducing or eliminating a major impediment to optimal utilization of precious liquid DNA, cDNA, and other precious liquid source materials which form the bedrock of research linking phenotype and genotype. Compared to present methods, this technology will greatly improve research efficiency by reducing contamination and labor costs in handling samples. The database interface will allow researchers to address interesting outlier samples much more easily during the research process, and permit them to address their complete sample sets more readily when planning experiments.

描述（由申请人提供）：通过使用来自具有已知特征的受试者的 DNA、cDNA 或其他珍贵液体的储存水样测试假设，推进了综合疾病表型和细胞基因型研究。许多生命科学实验室将数千个此类水样储存数年或数十年，而这些研究人员绝对依赖于准确的库存知识和已知的样品来源。目前用于储存这些珍贵液体样品的方法是临时的，容易受到污染和错误识别。这以多种方式降低了研究人员的生产力和满意度。该项目的目标是开发一种系统，用于在标准化容器中长期储存贵重液体，并具有机器可读的识别功能，以便对其内容物进行积极和明确的识别。不需要移液器侵入的自动精密分配器将在分配微升等份珍贵液体的同时确保样品的完整性。自动分配设备和实验室数据库之间的接口将保持准确的库存控制并允许改进实验计划。该系统将确保珍贵的液体样本不受污染、保持其浓度水平并得到正确、积极的鉴定，从而大大提高基因组研究的效率并降低错误率。完成后，该系统将通过向生命科学实验室、法医实验室和医院销售来实现商业化。本方案的技术创新点是标准化储存容器的专利设计；一种新颖且专有的方法，可无污染地分配 1 ul 液滴，以及容器识别方法和数据库接口协议。在该项目的第一阶段，将设计和建造具有机器可读 ID 的一次性容器原型（具体目标 1），将开发专有的新型滴剂分配技术（具体目标 2），以及用于之间通信的软件接口协议将规划系统和实验室数据库（具体目标 3）。每个原型制造完成后，都会在运行中的生命科学实验室中进行 alpha 测试。在第二阶段，将建造一个完全自动化的系统，可直接连接到用户实验室数据库。通过减少或消除对珍贵液体 DNA、cDNA 和其他珍贵液体源材料（构成连接表型和基因型研究的基石）最佳利用的主要障碍，公共卫生将直接受益于拟议工作的结果。与现有方法相比，该技术将通过减少处理样品的污染和劳动力成本来大大提高研究效率。数据库接口将使研究人员能够在研究过程中更轻松地处理有趣的异常样本，并允许他们在计划实验时更轻松地处理完整的样本集。