National Resources for Imaging Mass Spectrometry

国家成像质谱资源

• 批准号: 7117395
• 负责人: CLAUDE P LECHENE
• 金额: $ 131.63万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7117395
• 关键词: carbon; health science research; image enhancement; image processing; imaging /visualization /scanning; mass spectrometry; method development; nitrogen; radionuclides; radiotracer

DESCRIPTION (provided by applicant): The core of this 'Resource' is a novel type of secondary ion mass spectrometer, the Multi-Isotope Imaging Mass Spectrometer (MIMS). Secondary ion mass spectrometry is based upon the sputtering of a few atomic layers from the surface of a sample. During the ejection process, some atoms and clusters are spontaneously ionized. These 'secondary ions' are a characteristic of the composition of the analyzed surface. In a secondary ion mass spectrometer, the secondary ions are separated according to their mass, and a quantitative image of the distribution of the selected mass is formed. MIMS provides high mass separation (M/deltaM > 10,000), high spatial resolution (< 50 nm) and has the unique capability of simultaneously recording several atomic mass images. Of the utmost importance, MINIS makes it possible for the first time (and at the intracellular level) to simultaneously image the distribution and measure the accumulation of molecules labeled with any isotopes, in particular with stable isotopes, for example with 15N. Thus, MIMS allows one to study the localization, the accumulation and the turnover of proteins, fats, sugars and foreign molecules in cellular microdomains. The Resource collaborates with researchers to use the unique advantages of MIMS to bring original information in cell biology, physiology, physiopathology, biochemistry, immunology, transplantation, toxicology, stem cell and gene transfer research. MINIS offers a powerful and quantitative new method for studying intra and transcellular metabolic pathways, signal transduction, RNA and DNA expression and distribution, fatty acid transport, donor-receiver cellular trafficking, stem cell nesting and localization of drugs. Finally, the use of stable isotopes offers a world of labeling possibilities that were impossible with auto-radiography. It will revive and expand the use of tracers in humans. Instrumental development (brighter cesium primary ion source, iodine negative primary ion source, bipolar Cs+/Isource, preanalysis covering of the sample surface with cesium) will increase the resolution to 20nm, will allow sensitive measurements of calcium, alkali metals (Na, K), metals (metalloenzymes) and will allow analysis of the very first atomic layers at the cell surface. Study of secondary ion formation, development of automation and powerful data reduction software, development of standards, and development of methods for long term labeling of cells will increase the breadth of the Resource and will benefit the community of users. A workshop has been and will be organized every year. A Resource website displays a wealth of information to the budding community of users. A secure server allows exchanges of large reams of data between the Resource and users. A webserver for the community of users is being built and will be maintained by the Resource.

描述（由申请人提供）：此“资源”的核心是一种新型的二次离子质谱仪，多相关成像质谱仪（MIMS）。次级离子质谱法基于样品表面的一些原子层的溅射。在弹出过程中，某些原子和簇自发地离子化。这些“二级离子”是分析表面组成的特征。在次级离子质谱仪中，根据其质量分离次级离子，并形成了所选质量分布的定量图像。 MIMS提供高质量分离（M/Deltam> 10,000），高空间分辨率（<50 nm），并且具有同时记录几个原子质量图像的独特能力。最重要的是，Minis使得第一次（在细胞内水平）可以同时对分布和测量具有任何同位素标记的分子的积累，尤其是稳定的同位素，例如15N。因此，MIMS允许一个人研究细胞微区域中蛋白质，脂肪，糖和外国分子的定位，积累和周转。该资源与研究人员合作利用MIMS的独特优势带来了细胞生物学，生理学，生理病理学，生物化学，免疫学，移植，毒理学，干细胞和基因转移研究的原始信息。 MINI提供了一种强大而定量的新方法，用于研究和跨细胞代谢途径，信号转导，RNA和DNA表达和分布，脂肪酸转运，供体接收者的细胞运输，干细胞嵌套和药物的定位。最后，使用稳定的同位素提供了一个标记可能性的世界，而自动射线照相则是不可能的。它将恢复并扩大示踪剂在人类中的使用。仪器开发（较明亮的颅骨离子源，碘负初级离子源，双极CS+/iSource，用剖腹的覆盖样品表面的覆盖的覆盖片）将使分辨率提高到20nm，将允许对钙，碱金属（Na，k），金属（Metalloenzymes）的钙测量，并允许分析的分析。研究次要离子形成，自动化开发和强大的数据减少软件的开发，标准的发展以及用于长期标记细胞的方法的开发将增加资源的广度，并将使用户社区受益。每年都会组织一个研讨会。资源网站向崭露头角的用户社区显示了大量信息。安全服务器允许在资源和用户之间交换大量数据。正在构建用于用户社区的网络服务器，并将由资源维护。